Targeted RNA sequencing of adrenal zones using immunohistochemistry-guided capture of formalin-fixed paraffin-embedded tissue
Autor: | Juilee Rege, William E. Rainey, Chia Jen Liu, Aaron M. Udager, Samuel W. Plaska, Zhaoping Qin, Jessica E Baker |
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Rok vydání: | 2021 |
Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Tissue Fixation 030209 endocrinology & metabolism Biology Biochemistry Article Transcriptome 03 medical and health sciences 0302 clinical medicine Endocrinology medicine Humans Molecular Biology Paraffin Embedding Adrenal gland Adrenal cortex Sequence Analysis RNA Gene Expression Profiling RNA High-Throughput Nucleotide Sequencing Immunohistochemistry 030104 developmental biology medicine.anatomical_structure Zona glomerulosa Adrenal Cortex Adrenal medulla Zona reticularis |
Zdroj: | Mol Cell Endocrinol |
ISSN: | 1872-8057 |
Popis: | Adequate access to fresh or frozen normal adrenal tissue has been a primary limitation to the enhanced characterization of the adrenal zones via RNA sequencing (RNAseq). Herein, we describe the application of targeted RNAseq to formalin-fixed paraffin-embedded (FFPE) normal adrenal gland specimens. Immunohistochemistry (IHC) was used to visualize and guide the capture of the adrenocortical zones and medulla. Following IHC-based tissue capture and isolation of RNA, high-throughput targeted RNAseq highlighted clear transcriptomic differences and identified differentially expressed genes among the adrenal zones. Our data demonstrate the ability to capture FFPE adrenal zone tissue for targeted transcriptomic analyses. Future comparison of normal adrenal zones will improve our understanding of transcriptomic patterns and help identify potential novel pathways controlling zone-specific steroid production. |
Databáze: | OpenAIRE |
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