Mannan and phosphomannan from Kuraishia capsulata yeast
| Autor: | Andrey S. Dmitrenok, E. V. Kulakovskaya, Nikolay E. Nifantiev, Nadezhda E. Ustyuzhanina, Alexander S. Shashkov, Vladimir M. Menshov, Tatiana Kulakovskaya |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Magnetic Resonance Spectroscopy Polymers and Plastics Stereochemistry 030106 microbiology Disaccharide Polysaccharide 01 natural sciences Phosphates Mannans 03 medical and health sciences chemistry.chemical_compound Hydrolysis Materials Chemistry Glycosyl Mannan chemistry.chemical_classification 010405 organic chemistry Organic Chemistry Nuclear magnetic resonance spectroscopy Yeast 0104 chemical sciences chemistry Carbohydrate Sequence Phosphodiester bond Saccharomycetales |
| Zdroj: | Carbohydrate polymers. 181 |
| ISSN: | 1879-1344 |
| Popis: | Linear mannan and branched phosphomannan were identified as exopolysaccharides produced by Kuraishia capsulata yeast. Their structures were determined using nuclear magnetic resonance spectroscopy. The repeating unit of mannan was found to be a trisaccharide →6)-α-Manp-(1→2)-α-Manp-(1→2)-α-Manp-(1→, while the phosphomannan was shown to be built of β-Manp-(1→2)-α-Manp-(1 disaccharide blocks linked by phosphodiester bonds via C-1 and C-6 of the reducing unit. The production of both polysaccharides was shown to depend on the phosphate concentration in the culture medium. In the absence of phosphate, only mannan was obtained, while an excess of KH2PO4 led to the exclusive production of phosphomannan. Chemical depolymerisation of phosphomannan led to the formation of disaccharide β-Manp-(1→2)-(6-P)-Manp, representing the repeating unit of the hydrolysed polysaccharide. The treatment of the disaccharide with alkaline phosphatase resulted in the formation of disaccharide β-Manp-(1→2)-Manp. The latest products can be transformed into glycosyl donors applicable further in the synthesis of oligosaccharides related to Candida cell wall polysaccharides. |
| Databáze: | OpenAIRE |
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