Detection and purification of a recombinant human B lymphotropic virus (HHV-6) in the baculovirus expression system by limiting dilution and DNA dot-blot hybridization
| Autor: | Thomas Weber, Nancy T. Chang, Katherine Y.M. Chiu, Ming-Chiu Fung, Tse-Wen Chang |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
Microbiological Techniques
Recombination Genetic B-Lymphocytes Baculoviridae biology viruses Genetic Vectors Nucleic Acid Hybridization Dot blot Insect Viruses Transfection biology.organism_classification Recombinant virus Virology Molecular biology Virus law.invention Plasmid Cell culture law DNA Viral Recombinant DNA Humans Herpesviridae |
| Zdroj: | Journal of Virological Methods. 19:33-42 |
| ISSN: | 0166-0934 |
| DOI: | 10.1016/0166-0934(88)90005-5 |
| Popis: | A recombinant virus Ac373-HB was detected and purified from the transfection mixture of wild type and recombinant virus in the baculovirus expression system using a combination of limiting dilution and DNA dot-blot hybridization. This method allows for a quick and convenient way of detection and purification of recombinant virus without the need to use a plaque purification step. It is generally applicable to other expression systems besides the baculovirus system described here. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|