K-ras-2 G-C and G-T transversions correlate with DNA aneuploidy in colorectal adenomas
| Autor: | A. Rapallo, Elio Geido, Walter Giaretti, Natalija Pujic, Angela Di Vinci, Stefano Nigro, Mauro Risio |
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| Rok vydání: | 1995 |
| Předmět: |
Adenoma
Adult Male Molecular Sequence Data Aneuploidy Biology Polymerase Chain Reaction S Phase law.invention chemistry.chemical_compound law medicine Humans Point Mutation DAPI Codon Transversion Polymerase chain reaction Aged Aged 80 and over Base Sequence Hepatology Cell growth Point mutation Gastroenterology DNA Neoplasm Middle Aged Flow Cytometry medicine.disease Molecular biology Genes ras chemistry Female Colorectal Neoplasms DNA |
| Zdroj: | Gastroenterology. 108:1040-1047 |
| ISSN: | 0016-5085 |
| DOI: | 10.1016/0016-5085(95)90201-5 |
| Popis: | K-ras-2 mutations and DNA content heterogeneity represent early events of human colorectal tumor progression. The aim of the study was to investigate if specific K-ras-2 mutations in 58 human sporadic adenomas were correlated with DNA aneuploidization and cell proliferation.Multiparameter flow cytometry, based on scatter parameters and DNA content, was performed using 4,6-diamidino-2-phenilindole-2-hydrochloride-stained nuclei obtained from adenoma fragments with either mild-moderate or severe dysplasia. K-ras-2 polymerase chain reaction and spectrum analysis were performed using sorted DNA specific epithelial subclones.We detected six G-A transitions, and four G-C and two G-T transversions. The DNA aneuploid subclones were 25 with DNA index values in the near diploid region (DNA index1.3) for the vast majority of cases (80%). DNA aneuploidy among the mutated adenomas with G-A transitions was 1 of 6 (17%) and 6 of 6 (100%) among G-C and G-T transversions. Although DNA aneuploidy and high S-phase values were also present among K-ras-2 wild-type adenomas, their statistical associations with K-ras-2 status were P0.005 and P0.05, respectively.The present series of sporadic colorectal adenomas indicates that codon 12 G-C and G-T K-ras-2 transversion mutations and DNA aneuploidy are correlated. The underlying mechanisms that explain such association remain to be investigated. |
| Databáze: | OpenAIRE |
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