Single-cell derived clones from human adipose stem cells present different immunomodulatory properties
| Autor: | M. L. de la Sen, María I. Arribas, Juan A. Reig, Pascual Martínez-Peinado, Enrique Roche, Mario F. Fraga, Jose Zubcoff, José M. Sempere, Alfredo Santana, Agustín F. Fernández |
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| Přispěvatelé: | Universidad de Alicante. Departamento de Biotecnología, Universidad de Alicante. Departamento de Ciencias del Mar y Biología Aplicada, Grupo de Inmunología, Web and Knowledge (WaKe) |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
CD4-Positive T-Lymphocytes
CD3 Complex T-Lymphocytes Interleukin-1beta Immunology Clone (cell biology) Inmunología CD8-Positive T-Lymphocytes Biology Cell Clone Estadística e Investigación Operativa Cluster Analysis Humans Immunology and Allergy Cells Cultured Interleukin 4 Cell Proliferation Adult stem cells Inflammation Reverse Transcriptase Polymerase Chain Reaction Mesenchymal Stem Cells Original Articles Cell cloning DNA Methylation Flow Cytometry Molecular biology Coculture Techniques Clone Cells Cytokine secretion Killer Cells Natural Adipose Tissue Cell culture Cytokines Interleukin-4 Inflammation Mediators Interleukin-5 Stem cell Transcriptome DNA-methylation CD8 Adult stem cell |
| Zdroj: | RUA. Repositorio Institucional de la Universidad de Alicante Universidad de Alicante (UA) |
| Popis: | Human adipose mesenchymal stem cells are a heterogeneous population, where cell cultures derived from single cell-expanded clones present varying degrees of differential plasticity. This work focuses on the immunomodulatory/anti-inflammatory properties of these cells. To this end, 5 single cell clones were isolated (generally called 1.X and 3.X) from 2 volunteers. Regarding the expression level of the lineage-characteristic surface antigens, clones 1.10 and 1.22 expressed the lowest amounts, while clones 3.10 and 3.5 expressed more CD105 than the rest and clone 1.7 expressed higher amounts of CD73 and CD44. Regarding cytokine secretion, all clones were capable of spontaneously releasing high levels of IL-6 and low to moderate levels of IL-8. These differences can be explained in part by the distinct methylation profile exhibited by the clones. Furthermore and after lipopolysaccharide stimulation, clone 3.X produced the highest amounts of pro-inflammatory cytokines such as IL-1β, while clones 1.10 and 1.22 highly expressed IL-4 and IL-5. In co-culture experiments, clones 1.X are altogether more potent inhibitors than clones 3.X for proliferation of total, CD3+T, CD4+T and CD8+T lymphocytes and NK cells. The results of this work indicates that adipose stem cell population is heterogeneous in cytokine production profile, and that isolation, characterization and selection of the appropriate cell clone is a more exact method for the possible treatment of different patients or pathologies. Instituto de Salud Carlos IIIFEDER (PS09/01093), Fundacion Salud 2000-Merck Serono, Generalitat Valenciana (PROMETEO/2012/007) and “Centro de Investigación Biomédica en Red de Fisiopatología de la Obesidad y Nutrición” CIBERobn (CB12/03/30038) to ER, and VALi+d (APOSTD/2012/021) from Generalitat Valenciana to MIA. |
| Databáze: | OpenAIRE |
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