Modification of RNA Polymerase after T3 Phage Infection of Escherichia coli B
| Autor: | S. P. Mahadik, B. Dharmgrongartama, P.R. Srinivasan |
|---|---|
| Rok vydání: | 1973 |
| Předmět: |
T7 phage
Specificity factor RNA-dependent RNA polymerase Thymus Gland Coliphages Chromatography DEAE-Cellulose RNA polymerase III Epitopes chemistry.chemical_compound Transcription (biology) RNA polymerase Centrifugation Density Gradient Escherichia coli Animals T3 phage Carbon Radioisotopes Lysogeny Biological Sciences: Cell Biology Polymerase Immunoassay Chromatography Multidisciplinary biology DNA DNA-Directed RNA Polymerases Templates Genetic biology.organism_classification Molecular biology Molecular Weight chemistry biology.protein Cattle Electrophoresis Polyacrylamide Gel Rabbits |
| Zdroj: | Proceedings of the National Academy of Sciences. 70:2845-2849 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.70.10.2845 |
| Popis: | E. coli B cells infected with T3 phage contain a modified host RNA polymerase in addition to the normal RNA polymerase found in uninfected cells. The modified RNA polymerase behaves differently in its elution properties from the normal enzyme on DEAE-cellulose, phosphocellulose, and DNA-cellulose column chromatography. The modified enzyme also differs from the normal polymerase in some of its enzymatic parameters. The specific activity of the modified RNA polymerase is markedly lower (i.e., ¼) than that of the normal enzyme. The decrease in activity is probably due to an alteration in the β′ subunit of the polymerase. A similar modification is also observed in nonpermissive cells infected with a gene-l amber mutant incapable of producing active T3 specific polymerase. An analogous modification in host RNA polymerase does not seem to occur in E. coli cells infected with T7 phage. |
| Databáze: | OpenAIRE |
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