Poly(ε-caprolactone) Titanium Dioxide and Cefuroxime Antimicrobial Scaffolds for Cultivation of Human Limbal Stem Cells
| Autor: | Iva Čanak, Anita Tarbuk, Emilija Zdraveva, Marina Bujić Mihica, Tamara Dolenec, Igor Slivac, Mirna Tominac Trcin, Valentina Blažević, Antoneta Tomljenović, Budimir Mijović, Emi Govorčin Bajsić, Iva Dekaris, Nikolina Mrkonjić, Sunčica Kuzmić, Tamara Holjevac Grgurić, Ksenija Markov, Ivanka Batarilo, Ivana Vrgoč Zimić |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Polymers and Plastics
02 engineering and technology 010402 general chemistry 01 natural sciences Article lcsh:QD241-441 Tissue engineering lcsh:Organic chemistry polycaprolactone Cornea scaffolds tissue engineering electrospinning titanium dioxide cefuroxime limbal stem cell deficiency antimicrobial activity medicine Chemistry technology industry and agriculture General Chemistry Adhesion Eye infection 021001 nanoscience & nanotechnology Antimicrobial 0104 chemical sciences Transplantation medicine.anatomical_structure Surface modification sense organs Stem cell 0210 nano-technology Biomedical engineering |
| Zdroj: | Polymers Volume 12 Issue 8 Polymers, Vol 12, Iss 1758, p 1758 (2020) |
| ISSN: | 2073-4360 |
| DOI: | 10.3390/polym12081758 |
| Popis: | Limbal Stem Cell Deficiency (LSCD) is a very serious and painful disease that often results in impaired vision. Cultivation of limbal stem cells for clinical application is usually performed on carriers such as amniotic membrane or surgical fibrin gel. Transplantation of these grafts is associated with the risk of local postoperative infection that can destroy the graft and devoid therapeutic benefit. For this reason, electrospun scaffolds are good alternatives, as proven to mimic the natural cells surroundings, while their fabrication technique is versatile with regard to polymer functionalization and scaffolds architecture. This study considers the development of poly(&epsilon caprolactone) (PCL) immune-compatible and biodegradable electrospun scaffolds, comprising cefuroxime (CF) or titanium dioxide (TiO2) active components, that provide both bactericidal activity against eye infections and support of limbal stem cells growth in vitro. The PCL/CF scaffolds were prepared by blend electrospinning, while functionalization with the TiO2 particles was performed by ultrasonic post-processing treatment. The fabricated scaffolds were evaluated in regard to their physical structure, wetting ability, static and dynamic mechanical behaviour, antimicrobial efficiency and drug release, through scanning electron microscopy, water contact angle measurement, tensile testing and dynamic mechanical analysis, antimicrobial tests and UV-Vis spectroscopy, respectively. Human limbal stem cells, isolated from surgical remains of human cadaveric cornea, were cultured on the PCL/CF and PCL/TiO2 scaffolds and further identified through immunocytochemistry in terms of cell type thus were stained against p63 marker for limbal stem cells, a nuclear transcription factor and cytokeratin 3 (CK3), a corneal epithelial differentiation marker. The electrospun PCL/CF and PCL/TiO2 successfully supported the adhesion, proliferation and differentiation of the cultivated limbal cells and provided the antimicrobial effect against Pseudomonas aeruginosa, Staphylococcus aureus and Candida albicans. |
| Databáze: | OpenAIRE |
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