Disposable electrochemical immunosensor for Brettanomyces bruxellensis based on nanogold-reduced graphene oxide hybrid nanomaterial
| Autor: | Concepción Parrado, Abderrahmane Boujakhrout, María Arévalo-Villena, Boryana Borisova, Alfredo Sánchez, Reynaldo Villalonga, José M. Pingarrón, Maria L. Villalonga, Ana Briones-Pérez |
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| Rok vydání: | 2017 |
| Předmět: |
Oxide
Brettanomyces Metal Nanoparticles Brettanomyces bruxellensis Wine Nanotechnology 02 engineering and technology 01 natural sciences Biochemistry Analytical Chemistry law.invention Nanomaterials chemistry.chemical_compound Limit of Detection law Immunoassay Detection limit biology Chemistry Graphene 010401 analytical chemistry Reproducibility of Results Oxides Electrochemical Techniques 021001 nanoscience & nanotechnology biology.organism_classification Amperometry Nanostructures 0104 chemical sciences Graphite Gold 0210 nano-technology Antibodies Immobilized Oxidation-Reduction Biosensor Nuclear chemistry |
| Zdroj: | Analytical and Bioanalytical Chemistry. 409:5667-5674 |
| ISSN: | 1618-2650 1618-2642 |
| Popis: | The assembly of a novel disposable amperometric immunosensor for the detection of the red wine spoilage yeast Brettanomyces bruxellensis is reported. The nanostructured sensing interface was prepared by first coating carbon screen printed electrodes with a gold nanoparticles-reduced graphene oxide hybrid nanomaterial, which was then modified with 3-mercaptopropionic acid to further immobilize specific antibodies for B. bruxellensis via a carbodiimide-coupling reaction. The functionalized electrode allowed the amperometric detection of B. bruxellensis in buffered solutions and red wine samples in the range of 10–106 CFU/mL and 102–106 CFU/mL, with low detection limits of 8 CFU/mL and 56 CFU/mL, respectively. The electrochemical immunosensor also exhibited high reproducibility, selectivity, and storage stability. |
| Databáze: | OpenAIRE |
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