Discrimination among cultivars of rapeseed (Brassica napus L.) using DNA polymorphisms amplified from arbitrary primers

Autor:	R. Scarth, B. Fristensky, R. J. Mailer
Rok vydání:	1994
Předmět:	Rapeseed Breeding program fungi UPGMA Dna polymorphism Brassica food and beverages General Medicine Biology biology.organism_classification Botany Plant biochemistry Genetics Cultivar Band pattern Agronomy and Crop Science Biotechnology
Zdroj:	Theoretical and Applied Genetics. 87:697-704
ISSN:	1432-2242 0040-5752
DOI:	10.1007/bf00222895
Popis:	RAPDs (Randomly Amplified Polymorphic DNAs) were used to discriminate among 23 cultivars of oilseed rape (Brassica napus) selected from several breeding programs. A set of 100 random sequence 10-mer primers were tested, of which 70 produced bands and 22 showed evidence of polymorphism. A selection of six primers produced 23 polymorphic bands of between 300 to 2200 base pairs in size, sufficient to distinguish between the cultivars. An analysis of seed of five cultivars obtained from four different sites showed stability of banding pattern over source of seed. The analysis was repeated using four different thermocyclers, each of which produced the same band pattern. UPGMA cluster analysis indicates that the relationships among some of the cultivars is closer for those from the same breeding program than for those from different programs. The results of this study show that RAPDs can be used as a method of identification for oilseed rape cultivars.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d7e6dab6eec42479ae251922ba390491 https://doi.org/10.1007/bf00222895 Zobrazit plný text záznamu Full text from SpringerLink