Curcumin alleviates oxidative stress and inhibits apoptosis in diabetic cardiomyopathy via Sirt1‐Foxo1 and PI3K‐Akt signalling pathways
| Autor: | Xiao‐jing Cheng, Yuan‐yuan Tie, Min‐feng Hao, Xue‐yi Li, Xiao‐guang Cui, Xin Yang, Bin‐cheng Ren, Yu‐fei Zhang, Hui Zhao, Shan‐shan Liu, Xin‐rui Zhao, Li Qu, Meng‐dan Li |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Blood Glucose Male Curcumin Cell Survival Diabetic Cardiomyopathies FOXO1 Apoptosis Nerve Tissue Proteins Pharmacology medicine.disease_cause Diabetes Mellitus Experimental Rats Sprague-Dawley 03 medical and health sciences chemistry.chemical_compound Phosphatidylinositol 3-Kinases 0302 clinical medicine Sirtuin 1 Diabetes mellitus Diabetic cardiomyopathy medicine oxidative stress Animals Myocytes Cardiac Phosphorylation Protein kinase B PI3K/AKT/mTOR pathway Sirt1 Myocardium PI3K‐Akt Cell Biology Original Articles medicine.disease Rats 030104 developmental biology chemistry 030220 oncology & carcinogenesis Molecular Medicine Original Article type 2 diabetes Proto-Oncogene Proteins c-akt Oxidative stress Signal Transduction |
| Zdroj: | Journal of Cellular and Molecular Medicine |
| ISSN: | 1582-4934 1582-1838 |
| Popis: | Diabetes is a disorder of glucose metabolism, and over 90% are type 2 diabetes. Diabetic cardiomyopathy (DCM) is one of the type 2 diabetes complications, usually accompanied by changes in myocardial structure and function, together with cardiomyocyte apoptosis. Our study investigated the effect of curcumin on regulating oxidative stress (OS) and apoptosis in DCM. In vivo, diabetes was induced in an experimental rat model by streptozoticin (STZ) together with high‐glucose and high‐fat (HG/HF) diet feeding. In vitro, H9c2 cardiomyocytes were cultured with high‐glucose and saturated free fatty acid palmitate. Curcumin was orally or directly administered to rats or cells, respectively. Streptozoticin ‐induced diabetic rats showed metabolism abnormalities and elevated markers of OS (superoxide dismutase [SOD], malondialdehyde [MDA], gp91phox, Cyt‐Cyto C), enhanced cell apoptosis (Bax/Bcl‐2, Cleaved caspase‐3, TUNEL‐positive cells), together with reduced Akt phosphorylation and increased Foxo1 acetylation. Curcumin attenuated the myocardial dysfunction, OS and apoptosis in the heart of diabetic rats. Curcumin treatment also enhanced phosphorylation of Akt and inhibited acetylation of Foxo1. These results strongly suggest that apoptosis was increased in the heart of diabetic rats, and curcumin played a role in diabetic cardiomyopathy treatment by modulating the Sirt1‐Foxo1 and PI3K‐Akt pathways. |
| Databáze: | OpenAIRE |
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