| Autor: |
L V, Muscatello, G, Avallone, B, Brunetti, B, Bacci, M P, Foschini, G, Sarli |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
The Veterinary Journal. :105833 |
| ISSN: |
1090-0233 |
| DOI: |
10.1016/j.tvjl.2022.105833 |
| Popis: |
Neoplastic cells, through immunoediting mechanisms, can establish a state of immunosuppression to evade host immune defenses. The aims of this study were: (1) to validate a standard method for assessing tumor infiltrating lymphocytes (TILs) in canine mammary carcinoma by applying international human breast cancer guidelines; (2) to investigate if the TILs population was composed of a subset of regulatory T lymphocytes (Tregs); and (3) to evaluate the relationship between the number of TILs and Tregs and the biological behavior of the tumors. One hundred and twenty-nine canine mammary tumors were retrospectively selected for this study. Histological diagnosis, grading and histological evaluation of TILs was performed on hematoxylin and eosin-stained sections. TILs were evaluated using a three-tier semiquantitative method, previously validated in human medicine, based on the percentage of TILs (0-10%, 11-40% and 41-90%). Lymphocyte immunophenotype was confirmed by CD3 and CD79, while an anti-FoxP3 antibody was used to determine the presence of Tregs. The number of stromal TILs and invasive front TILs significantly correlated with each other (P0.0001) and increased with increasing histological grade (P = 0.002 and P = 0.004, respectively). A subset of TILs was composed of FOXP3 |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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Full Text from ScienceDirect