The nicotinic cholinergic system is affected in rats with delayed carbon monoxide encephalopathy
| Autor: | Yoko Mori, Shu-ichi Ueno, Cheng Li, Shinichiro Ochi, Yuta Yoshino, Ken-ichi Yoshida, Takaaki Mori, Takashi Ishimaru, Ryuji Fukuhara, Masao Abe, Kiyohiro Yamazaki, Satoshi Tanimukai, Seiji Matsuda |
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| Rok vydání: | 2013 |
| Předmět: |
Male
medicine.medical_specialty Pathology Programmed cell death Encephalopathy Hippocampus Cell Count Hippocampal formation Receptors Nicotinic Carbon Monoxide Poisoning Internal medicine medicine Avoidance Learning Reaction Time Animals Rats Wistar Acetylcholine receptor Neurons Carbon Monoxide biology Chemistry Carbon monoxide poisoning General Neuroscience CHRNA7 medicine.disease Nicotinic agonist Endocrinology biology.protein Neurotoxicity Syndromes |
| Zdroj: | Neuroscience letters. 569 |
| ISSN: | 1872-7972 |
| Popis: | Delayed carbon monoxide (CO) encephalopathy may occur following recovery from acute CO poisoning. However, the mechanism of delayed neuronal injury remains unknown. The nicotinic acetylcholine receptors (nAChRs) have been suggested to play a role in cognitive status in neurodegenerative diseases, including Alzheimer's disease. Therefore, in the current study, we investigated the effect of delayed neuronal CO poisoning on gene expression of nAChRs in the hippocampus of Wistar rats. Behavioral effects (measured by the passive-avoidance test) and histological analyses (hematoxylin-eosin-stained hippocampal cell counts and cell death observations) were also investigated, 21 days after CO exposure for 1h (1000ppm for 40min+3000ppm for 20min). Our findings show cognitive impairment and hippocampal cell death, suggesting our rat model is suitable for studying delayed CO encephalopathy. Expression of nAChR (Chrna3, Chrna4, Chnra7, and Chrnb2) mRNA was assessed using quantitative real-time polymerase chain reaction. Hippocampal Chrna3 expression was significantly decreased, and cerebellar Chrna7 expression significantly increased, in the delayed CO encephalopathy rat model. Thus, the nicotinic cholinergic system may be affected in delayed CO encephalopathy. |
| Databáze: | OpenAIRE |
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