Development of an itraconazole resistance gene as a dominant selectable marker for transformation in Aspergillus oryzae and Aspergillus luchuensis
| Autor: | Jikian Tokashiki, Hirohide Toyama, Osamu Mizutani |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Genetic Markers
0301 basic medicine Antifungal Agents Itraconazole Aspergillus oryzae Genes Fungal 030106 microbiology medicine.disease_cause Applied Microbiology and Biotechnology Biochemistry Genome Analytical Chemistry Microbiology 03 medical and health sciences Drug Resistance Fungal medicine Molecular Biology Gene Selectable marker Mutation biology Organic Chemistry General Medicine biology.organism_classification Transformation (genetics) Aspergillus 030104 developmental biology biology.protein Demethylase Biotechnology medicine.drug |
| Zdroj: | Bioscience, Biotechnology, and Biochemistry. 85:722-727 |
| ISSN: | 1347-6947 |
| DOI: | 10.1093/bbb/zbaa080 |
| Popis: | There are only a few combinations of antifungal drugs with known resistance marker genes in the Aspergillus species; therefore, the transformation of their wild-type strains is limited. In this study, to develop the novel dominant selectable marker for itraconazole, a fungal cell membrane synthesis inhibitor, we focused on Aspergillus luchuensis cyp51A (Alcyp51A), which encodes a 14-α-sterol demethylase related to the steroid synthesis pathway. We found that the G52R mutation in AlCyp51A and the replacement of the native promoter with a high-expression promoter contributed to itraconazole resistance in Aspergillus oryzae, designated as itraconazole resistant gene (itrA). The random integration in the A. luchuensis genome of the itrA marker cassette gene also allowed for transformation using itraconazole. Therefore, we succeed in developing a novel itraconazole resistance marker as a dominant selectable marker for transformation in A. oryzae and A. luchuensis. |
| Databáze: | OpenAIRE |
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