Complementation studies with Rous sarcoma virus gag and gag-pol polyprotein mutants
| Autor: | Suzanne Oertle, Pierre-François Spahr, Neil Edward Bowles |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
medicine.medical_treatment
viruses Immunology DNA Mutational Analysis Molecular Sequence Data Retroviridae Proteins Gene Products gag Gene Products pol Chick Embryo Microbiology Avian sarcoma virus Virus Retrovirus Protein-fragment complementation assay Virology Endopeptidases medicine Animals Rous sarcoma virus Protease biology Base Sequence Genetic Complementation Test Virion Group-specific antigen biology.organism_classification Molecular biology Fusion Proteins gag-pol Complementation Enzyme Activation Avian Sarcoma Viruses Insect Science Protein Processing Post-Translational Research Article |
| Zdroj: | Journal of virology. 66(6) |
| ISSN: | 0022-538X |
| Popis: | Avian retroviruses (with the notable exception of spleen necrosis virus) express their protease (PR) both in their gag and their gag-pol polyprotein precursors, in contrast to other retroviruses, notably, the mammalian retroviruses, in which PR is encoded in the gag-pol polyprotein or in a separate reading frame as a gag-pro product. The consequence is that the avian PR is expressed in stoichiometric rather than catalytic amounts. To investigate the significance of the particular genome organization of the avian retrovirus prototype Rous sarcoma virus, we developed an assay that measures complementation between the gag and the gag-pol polyproteins by expressing them from two different plasmids in transfected cells. By using this assay, we showed that the protease PR from the gag-pol polyprotein is capable of autocatalytic self-cleavage and -activation when coexpressed with a protease-deficient gag protein and that the PR domain has a role in viral particle assembly. Furthermore, this complementation assay can be used to investigate the role of the gag domain in the gag-pol polyprotein by determining whether it can rescue a defect in the gag polyprotein. We report here the results of such an experiment, which studied a mutation in the N terminus of the gag gene. |
| Databáze: | OpenAIRE |
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