Application of sensitive and specific molecular methods to uncover global dissemination of the major RDRio sublineage of the Latin american-mediterranean Mycobacterium tuberculosis spoligotype family
| Autor: | Andrea L. Gibson, Daniel W. Fitzgerald, Silvana Miranda Spindola, Richard C. Huard, Afrânio Lineu Kritski, Natalia Kurepina, Luiz Claudio Oliveira Lazzarini, Darío García de Viedma, Jose Lapa e Silva, Brigitte Gicquel, Poonam Chitale, Kristin Kremer, Dick van Soolingen, Robin M. Warren, Barry N. Kreiswirth, Thierry Zozio, Nicolaas C. Gey van Pittius, Jean W. Pape, Helmi Mardassi, Nalin Rastogi, John L. Ho, Christophe Sola, Jessica Brinkworth, Paul D. van Helden, Jeffrey Driscoll, Philip Noel Suffys |
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| Přispěvatelé: | Weill Medical College of Cornell University [New York], Columbia University Medical Center [New York], New York Presbyterian Hospital, DST/NRF Centre of Excellence in Strong Materials (Johannesburg, South Africa ), University of the Witwatersrand [Johannesburg] (WITS), Institute of Thoracic Disease [Rio de Janeiro], Federal University of Rio de Janeiro, Wadsworth Center, New York State Department of Health [Albany], Public Health Research Institute [Newark] (PHRI), Institut Pasteur de la Guadeloupe, Réseau International des Instituts Pasteur (RIIP), Génétique mycobactérienne - Mycobacterial genetics, Institut Pasteur [Paris], Universidade Federal de Minas Gerais [Belo Horizonte] (UFMG), GHESKIO Centers [Port-au-Prince], National Institute for Public Health and the Environment [Bilthoven] (RIVM), Institut Pasteur de Tunis, New York Consortium in Evolutionary Primatology (NYCEP), City University of New York [New York] (CUNY), Centro de Investigación Biomédica en Red Enfermedades Respiratorias (CIBERES), Servicio de Microbiología Clínica y Enfermedades Infecciosas, Hospital Gregorio Marañón, Rutgers Biomedical and Health Sciences, Rutgers University System (Rutgers), Fundação Oswaldo Cruz (FIOCRUZ), Funding for this project was provided by National Institutes of Health (NIH) grants R21 AI063147 and R21 AI063147 (to J.L.H.),NIH Fogarty International Center training grant (FICTG) (D43 TW00018), Innovative Approaches for TB Control in Brazil (Rio-ICOHRTA)-U2R TW006885 NIH, FIC (J.R.L.E.S., R. Chaisson, and J.L.H.), Haiti AIDS Research Training: Models to Implementation(Haiti-ICOHRTA)-U2R TW006901 (W. D. Johnson), a grant from the Coordenac ̧a ̃o de Aperfeicoamento de Pessoal de Nivel Superior(Ministry of Education of Brazil) from the Brazilian Ministry of Health (024/94 DST/AIDS), Fundacao Universita ria Jose Bonifacio/FUJB, the Brazilian Research Council/CNPq, the Brazilian Research Council/World Bank Millennium Institute of Science, the Programa de Apoio a Nu ́cleos de Excelencia, and PAPES IV/FIOCRUZ (P.N.S. and J.R.L.E.S.), and a grant from the Laura Cook Hull Trust Fund (LCHTF) (W. D. Johnson). A.L.G. was supported in part by the LCHTF, and L.C.O.L was a FICTG and is now a Rio-ICOHRTA and a CNPq trainee. The study performed in N.R.’s laboratory was partially funded by the European Regional Development Fund, European Commission (ERDF/FEDER, A34-05). T.Z. received a Ph.D. fellow-ship awarded by the European Union and the Regional Council of Guadeloupe and the International Network of Pasteur Institutes. Funding for the Spanish isolates was from the Fondo de Investigaciones Sanitarias (FIS030654, FIS060882) and the Instituto de Salud Carlos III (CIBER Enfermedades Respiratorias CIBERES, CB06/06/0058), Institut Pasteur [Paris] (IP), Fundação Oswaldo Cruz / Oswaldo Cruz Foundation (FIOCRUZ) |
| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
MESH: Mycobacterium tuberculosis
[SDV]Life Sciences [q-bio] Oligonucleotides MESH: Global Health MESH: Acyltransferases Global Health Polymerase Chain Reaction law.invention MESH: Mediterranean Region law MESH: Oligonucleotides hemic and lymphatic diseases MESH: Tuberculosis MESH: Phylogeny Phylogeny Polymerase chain reaction MESH: Evolution Molecular Genetics 0303 health sciences Phylogenetic tree MESH: Codon Mediterranean Region MESH: Polymorphism Single Nucleotide 3. Good health MESH: DNA Transposable Elements Restriction fragment length polymorphism MESH: Fatty Acid Synthases Microbiology (medical) Tuberculosis Single-nucleotide polymorphism Biology Polymorphism Single Nucleotide Sensitivity and Specificity Evolution Molecular Mycobacterium tuberculosis 03 medical and health sciences Multiplex polymerase chain reaction medicine Humans Codon 030304 developmental biology Antigens Bacterial MESH: Latin America MESH: Humans 030306 microbiology Mycobacteriology and Aerobic Actinomycetes MESH: Polymerase Chain Reaction medicine.disease biology.organism_classification bacterial infections and mycoses [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology MESH: Sensitivity and Specificity Latin America DNA Transposable Elements Bacterial antigen Fatty Acid Synthases Acyltransferases MESH: Antigens Bacterial |
| Zdroj: | Journal of Clinical Microbiology Journal of Clinical Microbiology, American Society for Microbiology, 2008, 46 (4), pp.1259-1267. ⟨10.1128/JCM.02231-07⟩ Journal of Clinical Microbiology, 2008, 46 (4), pp.1259-1267. ⟨10.1128/JCM.02231-07⟩ |
| ISSN: | 0095-1137 |
| DOI: | 10.1128/JCM.02231-07⟩ |
| Popis: | The Latin American-Mediterranean (LAM) family of Mycobacterium tuberculosis is believed to be the cause of ∼15% of tuberculosis cases worldwide. Previously, we defined a prevalent sublineage of the LAM family in Brazil by a single characteristic genomic deletion designated RD Rio . Using the Brazilian strains, we pinpoint an Ag85C 103 single nucleotide polymorphism (SNP) (screened by restriction fragment length polymorphism [RFLP] analysis) that correctly identified all LAM family strains. Importantly, all RD Rio strains concomitantly possessed the RD174 deletion. These genetic signatures, along with a newly developed multiplex PCR for rapid differentiation between “wild-type” and RD Rio strains, were then used to analyze an international collection of M. tuberculosis strains. RD Rio M. tuberculosis was identified from four continents involving 11 countries. Phylogenetic analysis of the IS 6110 -RFLP patterns from representative RD Rio and LAM strains from Brazil, along with all representative clusters from a South African database, confirmed their genetic relatedness and transcontinental transmission. The Ag85C 103 SNP RFLP, as compared to results obtained using a PCR method targeting a LAM-restricted IS 6110 element, correctly identified 99.8% of LAM spoligotype strains. Together, these tests were more accurate than spoligotyping at categorizing strains with indefinable spoligotypes and segregated true LAM strains from those with convergent spoligotypes. The fact that RD Rio strains were identified worldwide highlights the importance of this LAM family sublineage and suggests that this strain is a global threat that should be specifically targeted by public health resources. Our provision of simple and robust molecular methods will assist the evaluation of the LAM family and the RD Rio sublineage. |
| Databáze: | OpenAIRE |
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