Endothelial Colony Forming Cells as an Autologous Model to Study Endothelial Dysfunction in Patients with a Bicuspid Aortic Valve

Autor: Lidia R. Bons, Kirsten Lodder, Hans-Marc J. Siebelink, Kondababu Kurakula, Jolien W. Roos-Hesselink, Vera van de Pol, Marco C. DeRuiter, Marie-José Goumans, Gonzalo Sanchez-Duffhues
Přispěvatelé: Physiology, ACS - Pulmonary hypertension & thrombosis, Cardiology
Rok vydání: 2019
Předmět:
0301 basic medicine
Aortic valve
Male
endothelial colony forming cell
blood outgrowth endothelial cell
BOEC
Heart Valve Diseases
030204 cardiovascular system & hematology
migration
lcsh:Chemistry
calcification
0302 clinical medicine
Bicuspid aortic valve
Bicuspid Aortic Valve Disease
Cell Movement
Endothelial dysfunction
lcsh:QH301-705.5
Spectroscopy
Aorta
Cells
Cultured
General Medicine
Middle Aged
Computer Science Applications
medicine.anatomical_structure
Aortic Valve
Cardiology
cardiovascular system
Female
Aortic valve calcification
Dilatation
Pathologic
Adult
medicine.medical_specialty
bicuspid aortic valve
Catalysis
Article
Inorganic Chemistry
03 medical and health sciences
Young Adult
Internal medicine
medicine.artery
ECFC
medicine
Humans
Physical and Theoretical Chemistry
Aortic rupture
Molecular Biology
Cell Size
business.industry
Organic Chemistry
Endothelial Cells
BAV
medicine.disease
aortic dilation
030104 developmental biology
lcsh:Biology (General)
lcsh:QD1-999
business
Wound healing
Calcification
Zdroj: International Journal of Molecular Sciences, Vol 20, Iss 13, p 3251 (2019)
International Journal of Molecular Sciences, 20(13):3251. Multidisciplinary Digital Publishing Institute (MDPI)
International Journal of Molecular Sciences
Volume 20
Issue 13
International Journal of Molecular Sciences, 20(13). MDPI
van de Pol, V, Bons, L R, Lodder, K, Kurakula, K B, Sanchez-Duffhues, G, Siebelink, H M J, Roos-Hesselink, J W, Deruiter, M C & Goumans, M J 2019, ' Endothelial colony forming cells as an autologous model to study endothelial dysfunction in patients with a bicuspid aortic valve ', International Journal of Molecular Sciences, vol. 20, no. 13, 3251 . https://doi.org/10.3390/ijms20133251
ISSN: 1422-0067
1661-6596
DOI: 10.3390/ijms20133251
Popis: Bicuspid aortic valve (BAV), the most common congenital heart defect, is associated with an increased prevalence of aortic dilation, aortic rupture and aortic valve calcification. Endothelial cells (ECs) play a major role in vessel wall integrity. Little is known regarding EC function in BAV patients due to lack of patient derived primary ECs. Endothelial colony forming cells (ECFCs) have been reported to be a valid surrogate model for several cardiovascular pathologies, thereby facilitating an in vitro system to assess patient-specific endothelial dysfunction. Therefore, the aim of this study was to investigate cellular functions in ECFCs isolated from BAV patients. Outgrowth and proliferation of ECFCs from patients with BAV (n = 34) and controls with a tricuspid aortic valve (TAV, n = 10) were determined and related to patient characteristics. Interestingly, we were only able to generate ECFCs from TAV and BAV patients without aortic dilation, and failed to isolate ECFC colonies from patients with a dilated aorta. Analyzing EC function showed that while proliferation, cell size and endothelial-to-mesenchymal transition were similar in TAV and BAV ECFCs, migration and the wound healing capacity of BAV ECFCs is significantly higher compared to TAV ECFCs. Furthermore, calcification is blunted in BAV compared to TAV ECFCs. Our results reveal ECs dysfunction in BAV patients and future research is required to unravel the underlying mechanisms and to further validate ECFCs as a patient-specific in vitro model for BAV.
Databáze: OpenAIRE
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