Rapid Bedside Inactivation of Ebola Virus for Safe Nucleic Acid Tests
| Autor: | Gunnel Lindegren, Susanne Gjeruldsen Dudman, Malin Lundahl Stoltz, Anne-Marte Bakken Kran, Karoline Bragstad, Cristiano Salata, Ali Mirazimi, Anders Fomsgaard, Helen Karlberg, Maiken Worsøe Rosenstierne |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Lysis Time Factors viruses Point-of-Care Systems medicine.disease_cause Specimen Handling 03 medical and health sciences Virology Journal Article diagnostics Medicine Humans Viral rna inactivation ebola virus Ebola virus business.industry Ebola virus RNA Temperature RNA Blood collection Hemorrhagic Fever Ebola 3. Good health Disinfection 030104 developmental biology ebola virus inactivation diagnostics Nucleic acid RNA Viral Virus Inactivation business Nucleic acid detection |
| Zdroj: | Journal of Clinical Microbiology Rosenstierne, M W, Karlberg, H, Bragstad, K, Lindegren, G, Stoltz, M L, Salata, C, Kran, A-M B, Dudman, S G, Mirazimi, A & Fomsgaard, A 2016, ' Rapid Bedside Inactivation of Ebola Virus for Safe Nucleic Acid Tests ', Journal of Clinical Microbiology, vol. 54, no. 10, pp. 2521-2529 . https://doi.org/10.1128/JCM.00346-16 |
| DOI: | 10.1128/JCM.00346-16 |
| Popis: | Rapid bedside inactivation of Ebola virus would be a solution for the safety of medical and technical staff, risk containment, sample transport, and high-throughput or rapid diagnostic testing during an outbreak. We show that the commercially available Magna Pure lysis/binding buffer used for nucleic acid extraction inactivates Ebola virus. A rapid bedside inactivation method for nucleic acid tests is obtained by simply adding Magna Pure lysis/binding buffer directly into vacuum blood collection EDTA tubes using a thin needle and syringe prior to sampling. The ready-to-use inactivation vacuum tubes are stable for more than 4 months, and Ebola virus RNA is preserved in the Magna Pure lysis/binding buffer for at least 5 weeks independent of the storage temperature. We also show that Ebola virus RNA can be manually extracted from Magna Pure lysis/binding buffer-inactivated samples using the QIAamp viral RNA minikit. We present an easy and convenient method for bedside inactivation using available blood collection vacuum tubes and reagents. We propose to use this simple method for fast, safe, and easy bedside inactivation of Ebola virus for safe transport and routine nucleic acid detection. |
| Databáze: | OpenAIRE |
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