Carbon dioxide effects on olfactory functioning: behavioral, histological and immunohistochemical measurements
| Autor: | Grégory Pourié, G. Buron, Romain Hacquemand, Gérard Brand |
|---|---|
| Přispěvatelé: | Laboratoire de Neurosciences Intégratives et Cliniques - UFC ( NEURO ), Université Bourgogne Franche-Comté ( UBFC ) -Université de Franche-Comté ( UFC ), Laboratoire de Neurosciences Intégratives et Cliniques - UFC (EA 481) (NEURO), Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC) |
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Pathology
MESH : Olfactory Marker Protein MESH: Olfactory Perception MESH: Air Pollutants MESH : Immunohistochemistry MESH : Dose-Response Relationship Drug Toxicology MESH: Carbon Dioxide MESH : Behavior Animal Epithelium MESH: Dose-Response Relationship Drug MESH : Maze Learning Mice 0302 clinical medicine MESH: Behavior Animal MESH: Olfactory Marker Protein MESH: Animals MESH : Female MESH : Olfactory Bulb 0303 health sciences Air Pollutants Inhalation Exposure MESH : Mice Inbred Strains biology Inhalation Behavior Animal General Medicine Immunohistochemistry Olfactory Bulb MESH : Carbon Dioxide Sensory Thresholds [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] Female MESH: Inhalation Exposure MESH: Olfactory Bulb medicine.medical_specialty Period (gene) MESH : Air Pollutants Mice Inbred Strains Olfaction MESH: Mice Inbred Strains Andrology 03 medical and health sciences Olfactory Marker Protein Proliferating Cell Nuclear Antigen MESH : Mice MESH : Olfactory Perception medicine Animals Maze Learning MESH: Mice 030304 developmental biology Dose-Response Relationship Drug MESH: Maze Learning Histology MESH: Immunohistochemistry Carbon Dioxide Olfactory Perception MESH : Epithelium Proliferating cell nuclear antigen MESH: Epithelium MESH: Proliferating Cell Nuclear Antigen Odor 13. Climate action MESH : Proliferating Cell Nuclear Antigen [ SDV.NEU ] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] biology.protein MESH : Animals MESH : Inhalation Exposure MESH: Sensory Thresholds Olfactory marker protein MESH: Female 030217 neurology & neurosurgery MESH : Sensory Thresholds |
| Zdroj: | Toxicology Letters Toxicology Letters, Elsevier, 2009, 188 (3), pp.251-7. 〈10.1016/j.toxlet.2009.04.018〉 Toxicology Letters, Elsevier, 2009, 188 (3), pp.251-7. ⟨10.1016/j.toxlet.2009.04.018⟩ |
| ISSN: | 0378-4274 |
| Popis: | International audience; Most studies on toxic inhalation focus on solvent effects and few have dealt with gases on olfactory functioning. Among gases, the effects of carbon dioxide on general physiology have been well investigated contrary to the impact on olfactory neuroepithelium. Thus, this work was designed to evaluate in mice the possible effects of 3% CO(2) in two exposure periods: a 5h/day and a 12h/day conditions. Behavioral, histological and immunohistochemical observations were conducted every 2 weeks, i.e. before (W0), during (W2, W4) and after exposure (W6, W8). Firstly, behavioral evaluations of odor sensitivity showed differences in relation to the odor tested, i.e. no effect with congener urine odor and a reinforcement of 2,4,5-trimethythiazoline (TMT) (predator odor) repulsion. Secondly, histological evaluations showed a similar evolution of the epithelium thickness, i.e. a decrease along the exposure as well as during the post-exposure period and an increase of cell number (whatever the phenotype) although the kinetic appeared different in both experimental conditions. Thirdly, immunohistochemical quantification of olfactory marker protein (OMP)- and proliferating cell nuclear antigen (PCNA)-positive cells revealed that the number of mature olfactory neurons increased at the early beginning of exposure period in both conditions. While a decrease was observed in the following weeks (W4-W8) for the 12h/day condition, a stable amount of OMP-positive cells was maintained in the 5h/day condition. In contrast, the number of PCNA-positive cells followed a similar evolution, i.e. a constant decrease along the experiment. These findings indicate that the effects of CO(2) inhalation exposure are selectively dose-dependent. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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