Base Editing: The Ever Expanding Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) Tool Kit for Precise Genome Editing in Plants
| Autor: | Shaoqing Tang, Peisong Hu, Mahmuda Binte Monsur, Xiangjin Wei, Gaoneng Shao, Yusong Lv, Shakeel Ahmad |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine lcsh:QH426-470 Guanine Computational biology Review Biology 01 natural sciences base editor 03 medical and health sciences chemistry.chemical_compound Genome editing base conversion Genetics CRISPR genome editing Gene Genetics (clinical) Plant Proteins Gene Editing Cas9 Point mutation food and beverages Plants Thymine lcsh:Genetics 030104 developmental biology chemistry CRISPR-Cas Systems point mutation Genetic Engineering Cytosine clustered regularly interspaced short palindromic repeat 010606 plant biology & botany |
| Zdroj: | Genes, Vol 11, Iss 466, p 466 (2020) Genes |
| ISSN: | 2073-4425 |
| Popis: | Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR associated protein 9 (Cas9), a newly developed genome-editing tool, has revolutionized animal and plant genetics by facilitating modification of target genes. This simple, convenient base-editing technology was developed to improve the precision of genome editing. Base editors generate precise point mutations by permanent base conversion at a specific point, with very low levels of insertions and deletions. Different plant base editors have been established by fusing various nucleobase deaminases with Cas9, Cas13, or Cas12a (Cpf1), proteins. Adenine base editors can efficiently convert adenine (A) to guanine (G), whereas cytosine base editors can convert cytosine (C) to thymine (T) in the target region. RNA base editors can induce a base substitution of A to inosine (I) or C to uracil (U). In this review, we describe the precision of base editing systems and their revolutionary applications in plant science; we also discuss the limitations and future perspectives of this approach. |
| Databáze: | OpenAIRE |
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