Oxidative Stress Parameters in Goitrogen-Exposed Crested Newt Larvae (Triturus spp.): Arrested Metamorphosis
| Autor: | Caterina Faggio, Tijana B. Radovanović, Branka R. Gavrilović, Jelena P. Gavrić, Tamara G. Petrović, Svetlana G. Despotović, Marko D. Prokić, Tijana Vučić, Maja Ajduković, Milena Cvijanović |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
medicine.medical_specialty animal structures antioxidative compounds Health Toxicology and Mutagenesis media_common.quotation_subject medicine.disease_cause 010603 evolutionary biology 01 natural sciences Lipid peroxidation 03 medical and health sciences chemistry.chemical_compound Internal medicine medicine Metamorphosis Goitrogen thiourea 030304 developmental biology media_common thyroid hormones 0303 health sciences Larva amphibians biology Chemistry metamorphosis Public Health Environmental and Occupational Health biology.organism_classification Triturus Endocrinology Thiourea Catalase biology.protein Medicine Oxidative stress |
| Zdroj: | International Journal of Environmental Research and Public Health, Vol 18, Iss 9653, p 9653 (2021) International Journal of Environmental Research and Public Health Volume 18 Issue 18 |
| ISSN: | 1661-7827 1660-4601 |
| Popis: | Thiourea is an established disruptor of thyroid hormone synthesis and is frequently used as an inhibitor of metamorphosis. The changes caused by thiourea can affect processes associated with the oxidative status of individuals (metabolic rate, the HPI axis, antioxidant system). We investigated the parameters of oxidative stress in crested newt (Triturus spp.) larvae during normal development in late larval stage 62 and newly metamorphosed individuals, and during thiourea-stimulated metamorphosis arrest in individuals exposed to low (0.05%) and high (0.1%) concentrations of thiourea. Both groups of crested newts exposed to thiourea retained their larval characteristics until the end of the experiment. The low activities of antioxidant enzymes and the high lipid peroxidation level pointed to increased oxidative stress in larvae at the beginning of stage 62 as compared to fully metamorphosed individuals. The activities of catalase (CAT) and glutathione-S-transferase (GST) and the concentration of sulfhydryl (SH) groups were significantly lower in larvae reared in aqueous solutions containing thiourea than in newly metamorphosed individuals. The high thiourea concentration (0.1%) affected the antioxidative parameters to the extent that oxidative damage could not be avoided, contrary to a lower concentration. Our results provide a first insight into the physiological adaptations of crested newts during normal development and simulated metamorphosis arrest. |
| Databáze: | OpenAIRE |
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