Plasmid encoded β-lactamases resistant to inhibition by clavulanic acid produced by calf faecal coliforms
| Autor: | Alexander G. McLennan, J. N. Fletcher, C. A. Hart, John E. Corkill, J.E.B. Hunter |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Tetracycline
R Factors Microbial Sensitivity Tests Biology Amoxicillin-Potassium Clavulanate Combination medicine.disease_cause Apramycin beta-Lactamases Microbiology Clavulanic Acids Feces Plasmid Enterobacteriaceae Clavulanic acid Escherichia coli medicine Animals Humans Beta-Lactamase Inhibitors Antibacterial agent General Veterinary Amoxicillin Drug Resistance Microbial biochemical phenomena metabolism and nutrition biology.organism_classification Citrobacter freundii bacteria Cattle beta-Lactamase Inhibitors medicine.drug |
| Zdroj: | Research in Veterinary Science. 55:367-370 |
| ISSN: | 0034-5288 |
| DOI: | 10.1016/0034-5288(93)90109-s |
| Popis: | Two new plasmid encoded beta-lactamase enzymes produced by a strain of Escherichia coli and a strain of Citrobacter freundii isolated from calf faeces have been characterised. Both enzymes were similar to TEM-1 in terms of substrate and inhibition profiles and physical properties but differed from TEM-1 in being far less susceptible to the beta-lactamase inhibitors clavulanic acid or tazobactam. In each case transfer of the plasmid E coli K12 rendered it clinically resistant to the combination of amoxycillin and clavulanic acid. The beta-lactamase from the E coli had an iso-electric point (pI) of 5.4 and was encoded on a plasmid of 95 Kbp which also mediated resistance to tetracycline, sulphonamides, apramycin, streptomycin and gentamicin. The beta-lactamase from the C freundii had a pI of 5.2 and was encoded on a 75 Kbp plasmid which also mediated resistance to trimethoprim, chloramphenicol, apramycin, gentamicin and tobramycin. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|