HLA class II alleles associations of anticardiolipin and anti-beta2GPI antibodies in a large series of European patients with systemic lupus erythematosus
| Autor: | M, Galeazzi, G D, Sebastiani, A, Tincani, J C, Piette, F, Allegri, G, Morozzi, F, Bellisai, R, Scorza, G B, Ferrara, C, Carcassi, J, Font, G, Passiu, J, Smolen, C, Papasteriades, F, Houssiau, A F, Nebro, E D, Ramon Garrido, A, Jedryka-Goral, R, Marcolongo |
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| Rok vydání: | 2000 |
| Předmět: |
musculoskeletal diseases
Adult Male Adolescent Enzyme-Linked Immunosorbent Assay 030204 cardiovascular system & hematology Polymerase Chain Reaction 03 medical and health sciences 0302 clinical medicine Rheumatology immune system diseases HLA-DQ Antigens Humans Lupus Erythematosus Systemic skin and connective tissue diseases Alleles Aged Glycoproteins 030203 arthritis & rheumatology Histocompatibility Testing DNA HLA-DR Antigens Middle Aged Europe Immunoglobulin Isotypes beta 2-Glycoprotein I Antibodies Anticardiolipin Female DNA Probes |
| Zdroj: | Lupus. 9(1) |
| ISSN: | 0961-2033 |
| Popis: | The objective of this study was to determine the HLA class II associations of the anticardiolipin (aCL) and anti-b2GPI (ab2GPI) antibodies in a large series of European patients with systemic lupus erythematosus (SLE). A cohort of 577 European SLE patients was enrolled. aCL and ab2GPI were measured by ELISA methods. Molecular typing of HLA-DRB1, DRB3, DRB4, DRB5, DQA1 and DQB1 loci was performed by the polymerase chain reaction–sequence specific oligonucleotide probes (PCR–SSOP) method. aCL of IgG, IgM and IgA isotypes were detected in 22.8%, 14% and 13.9% of patients, respectively. IgG and IgM ab2GPI were detected in 20% of patients. aCL showed positive association with HLA DRB1*04, DRB1*0402, DRB1*0403, DRB1*07, DRB3*0301, DQA1*0201, DQA1*0301, DQB1*0302, and negative association with DQA1*0501, DRB3*0202. ab2GPI showed positive association with DRB1*0402, DRB1*0403, DQB1*0302. DRB1*0402 carried the highest relative risk for the presence of both aCL (RR = 8.1) and ab2GPI (RR = 4.6). Our results confirm the already described associations of aCL with HLA DR4 and DR7, but also demonstrate that, among the alleles at the DRB1*04 locus, the *0402 was most represented both in aCL and in ab2GPI positive patients. In addition, HLA class II associations of ab2GPI are for the first time extensively examined in a large cohort of European SLE patients. |
| Databáze: | OpenAIRE |
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