Alteration of gonadotropin-releasing hormone receptor expression with the progression of prostate cancer in the Dunning rat adenocarcinoma sublines
| Autor: | Anders Bergh, Åse Tieva, Jan-Erik Damber |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Male
endocrine system medicine.medical_specialty Blotting Western Adenocarcinoma urologic and male genital diseases Prostate cancer Cell Line Tumor Internal medicine medicine Animals Radiology Nuclear Medicine and imaging RNA Messenger Anaplasia Reverse Transcriptase Polymerase Chain Reaction business.industry Prostate Prostatic Neoplasms Hematology General Medicine medicine.disease Rats Gene Expression Regulation Neoplastic Disease Models Animal Endocrinology Oncology Hormone receptor Disease Progression Rat Adenocarcinoma business Receptors LHRH hormones hormone substitutes and hormone antagonists Gonadotropin-releasing hormone receptor |
| Zdroj: | Acta Oncologica. 44:299-303 |
| ISSN: | 1651-226X 0284-186X |
| DOI: | 10.1080/02841860510007512 |
| Popis: | Inhibitory effects of GnRH analogues on tumour growth in vitro suggests that such direct effects may be of importance also in vivo. However, the role of GnRH receptors (GnRH-R) in prostate tumour progression is largely unknown. The aim was therefore to investigate the variation of GnRH-R expression with prostate tumour progression using Dunning rat adenocarcinoma sublines representing different prostate tumour grades. GnRH-R levels were quantified in the rat dorsolateral (DLP) and Dunning sublines (PAP, AT-1, AT-2, AT-3, MatLyLu) using competitive RT-PCR and Western blot. The results showed that all Dunning sublines had significantly elevated GnRH-R mRNA expression levels compared with DLP. Comparison of GnRH-R mRNA levels between different tumour grades revealed no difference in mRNA expression. However, the anaplastic and highly metastatic AT-3 and MatLyLu tumours displayed a tendency for lower GnRH-R mRNA values than the non-metastatic tumour sublines. Our data demonstrate the expression of GnRH-R in normal rat DLP and in different Dunning sublines. However, GnRH-R seems not to be involved in tumour progression. |
| Databáze: | OpenAIRE |
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