Differential accumulation of monolignol-derived compounds in elicited flax (Linum usitatissimum) cell suspension cultures

Autor: Stéphane Maury, David Crônier, Mohamed Addi, Christophe Hano, Joël Doussot, Frédéric Lamblin, Eric Lainé, Sylvie Baltora-Rosset, Brigitte Chabbert, Lamine Bensaddek, François Mesnard, Simon Hawkins
Přispěvatelé: Laboratoire de Biologie des Ligneux et des Grandes Cultures (LBLGC), Institut National de la Recherche Agronomique (INRA)-Université d'Orléans (UO), Institut francilien recherche, innovation et société (IFRIS), Institut National de la Recherche Agronomique (INRA)-École des hautes études en sciences sociales (EHESS)-OST-Université Paris-Est Marne-la-Vallée (UPEM)-Ministère de l'Education nationale, de l’Enseignement supérieur et de la Recherche (M.E.N.E.S.R.)-ESIEE Paris-Centre National de la Recherche Scientifique (CNRS), Biologie des Plantes et Innovation - UR UPJV 3900 (BIOPI), Université de Picardie Jules Verne (UPJV), Fractionnement des AgroRessources et Environnement (FARE), Université de Reims Champagne-Ardenne (URCA)-Institut National de la Recherche Agronomique (INRA), Université de Picardie Jules Verne (UPJV)-Transfrontalière BioEcoAgro - UMR 1158 (BioEcoAgro), Université d'Artois (UA)-Université de Liège-Université de Picardie Jules Verne (UPJV)-Université du Littoral Côte d'Opale (ULCO)-Université de Lille-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-JUNIA (JUNIA), Université catholique de Lille (UCL)-Université catholique de Lille (UCL)-Université d'Artois (UA)-Université de Liège-Université du Littoral Côte d'Opale (ULCO)-Université de Lille-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-JUNIA (JUNIA), Université catholique de Lille (UCL)-Université catholique de Lille (UCL)
Rok vydání: 2005
Předmět:
0106 biological sciences
DOWN-REGULATION
Linum
Coumaric Acids
Plant Science
Phenylalanine ammonia-lyase
CINNAMYL-ALCOHOL-DEHYDROGENASE
FUSARIUM-OXYSPORUM
Lignin
01 natural sciences
Lignans
[SDV.GEN.GPL]Life Sciences [q-bio]/Genetics/Plants genetics
Biological Factors
03 medical and health sciences
chemistry.chemical_compound
WALL-BOUND PHENOLICS
Ascomycota
Fusarium
Glucosides
Phenols
Cell Wall
Flax
Spectroscopy
Fourier Transform Infrared
Genetics
Butylene Glycols
Cells
Cultured
030304 developmental biology
Lignan
0303 health sciences
TOBACCO NICOTIANA-TABACUM
CALLUS-CULTURES
Mycelium
biology
fungi
food and beverages
biology.organism_classification
Elicitor
chemistry
Sinapyl alcohol
Biochemistry
PHENYLPROPANOID BIOSYNTHESIS
ARABIDOPSIS-THALIANA
PLANT DEFENSE
Botrytis
Monolignol
EUCALYPTUS-GUNNII
010606 plant biology & botany
Coniferyl alcohol
Zdroj: Planta
Planta, Springer Verlag, 2006, 223 (5), pp.975-989. ⟨10.1007/s00425-005-0156-1⟩
Planta, 2006, 223 (5), pp.975-989. ⟨10.1007/s00425-005-0156-1⟩
ISSN: 1432-2048
0032-0935
Popis: International audience; Lignin and lignans share monolignols as common precursors and are both potentially involved in plant defence against pathogens. In this study, we investigated the effects of fungal elicitors on lignin and lignan metabolism in flax (Linum usitatissimum) cell suspensions. Cell suspension cultures of flax were treated with elicitor preparations made from mycelium extracts of Botrytis cinerea, Phoma exigua and Fusarium oxysporum F ssp lini. Elicitors induced a rapid stimulation of the monolignol pathway, as confirmed by the increase in PAL (phenylalanine ammonia-lyase, EC 4.1.3.5), CCR (cinnamoyl-CoA reductase EC 1.2.1.44) and CAD (cinnamyl alcohol dehydrogenase EC 1.1.1.195) gene expression and PAL activity. At the same time, CCR activity only increased significantly in F. oxysporum-treated cells 24 h post elicitation. On the other hand, CAD activity measured for coniferyl alcohol formation was transiently decreased but a substrate-specific activation of CAD activity was observed in F. oxysporum-treated cells when using sinapyl alcohol as substrate. The accumulation of monolignol-derived products varied according to the elicitor used. B. cinerea or P. exigua-elicited cell cultures were characterised by a reinforcement of the cell wall by a deposit of 8-O-4'-linked non-condensed lignin structures and phenolic monomers, while at the same time no stimulation of 8-8'-linked lignan or 8-5'-linked phenylcoumaran lignan accumulation was observed. Additionally, elicitation of cell cultures with F. oxysporum extracts even triggered a strong incorporation of monolignols in the non condensed labile ether-linked lignin fraction concomitantly with a decrease in lignan and phenylcoumaran lignan accumulation. Several hypotheses are proposed to explain the putative role of these compounds in the defence response of flax cells against pathogens.
Databáze: OpenAIRE
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