Rapid and Sensitive CZE Method for Quality Control Analysis of Pharmaceuticals Containing Pseudoephedrine, Triprolidine and Paracetamol
| Autor: | Sonia di Berardino, Renata Jasionowska |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Detection limit
Chromatography paracetamol Chemistry Hydrochloride pseudoephedrine triprolidine hydrochloride capillary zone electrophoresis quality control Repeatability Pseudoephedrine Psychiatry and Mental health Electrophoresis chemistry.chemical_compound Capillary electrophoresis medicine Pseudoephedrine Hydrochloride Triprolidine medicine.drug |
| Zdroj: | American Journal of Analytical Chemistry. :613-619 |
| ISSN: | 2156-8278 2156-8251 |
| DOI: | 10.4236/ajac.2014.59069 |
| Popis: | A rapid and sensitive CZE (Capillary Zone Electrophoresis) method for pharmaceutical analysis was developed and fully validated. The active compounds: Pseudoephedrine hydrochloride (PSE), Triprolidine hydrochloride (TRI) and Paracetamol (PAR) were separated and quantitatively determined using the tris-borate 30 mM buffer at pH = 9.0 as a Background Electrolyte (BGE). The electrophoretic separation was carried out at 25 kV in an unmodified fused silica capillary of I.D. = 50 μm with a “bubble-cell” for UV detection at 210 nm and 25°C. The separation was reached in about 3 min. After calibration the method was applied for analysis of three commercially available pharmaceutical preparations. The repeatability (RSD%) of migration time (tm) was ranging between 0.47% and 0.90% and of peak areas (A) between 0.63% and 3.64%. The Limit of Detection (LOD) values was of 0.19 μg/mL, 0.31 μg/mL and 0.08 μg/mL for respectively PSE, TRI and PAR. The results obtained in this study showed that the proposed method was useful in routinely analysis of pharmaceuticals. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|