Examination of tracheal allografts after long-term survival in dogs
| Autor: | Yongxing Zhang, Yu Liu, Yulei Qiao, Tao Lu, Yiwei Huang |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Pulmonary and Respiratory Medicine
Pathology medicine.medical_specialty medicine.medical_treatment 030230 surgery Revascularization Chondrocyte 03 medical and health sciences Chondrocytes Dogs 0302 clinical medicine Fibrosis Submucosa Bronchoscopy medicine Animals Transplantation Homologous business.industry Cartilage Mucous membrane General Medicine Allografts medicine.disease Trachea Transplantation medicine.anatomical_structure 030220 oncology & carcinogenesis Surgery Cardiology and Cardiovascular Medicine business Allotransplantation |
| Zdroj: | European Journal of Cardio-Thoracic Surgery. 59:155-161 |
| ISSN: | 1873-734X 1010-7940 |
| Popis: | OBJECTIVES Little is known on the outcome of tracheal allografts after long-term survival. This study aimed to explore the changes in structure and composition by evaluating the status of the mucosa and cartilage of allografts with long-term survival in dogs. METHODS Eight tracheal allografts that survived for ˃9 months were enrolled in our study. Epithelium, revascularization, monocyte infiltration and fibrosis were evaluated histologically. The fluorescent dye 4′-6-diamidino-2-phenylindole was used to evaluate the presence of chondrocyte nuclei. Glycosaminoglycan was detected using safranin-O staining and collagen II was evaluated using immunohistochemistry. RESULTS The 8 animals survived from 277 to 783 days. Bronchoscopy demonstrated that 6 allografts showed no stenosis; 2 cases developed slight stenosis, but could maintain airway patency. Histological examination showed that the epithelium covered the surface of the allografts. In comparison to fresh tracheal controls, allografts demonstrated mild monocyte infiltration, evident revascularization and mild fibrosis in the mucosa or submucosa (all P CONCLUSIONS For tracheal allografts with long-term survival after transplantation, only a few viable chondrocytes were retained, and the extracellular matrix of the cartilage demonstrated degeneration. Despite this, the airway could maintain patency. Notably, the significance of monocyte infiltration in the mucosa or submucosa at different time points warrants further study. |
| Databáze: | OpenAIRE |
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