Effect of Gabapentin/Memantine on the Infantile Nystagmus Syndrome in the Zebrafish Model: Implications for the Therapy of Ocular Motor Diseases
| Autor: | Maresa Afthinos, Stefan Yu Bögli, Melody Ying-Yu Huang |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
genetic structures
Gabapentin Cyclohexanecarboxylic Acids Eye Movements Ocular motor Nystagmus 03 medical and health sciences translational medicine 0302 clinical medicine Memantine Medicine Animals Spontaneous nystagmus Amines memantine zebrafish infantile nystagmus syndrome gabapentin Zebrafish gamma-Aminobutyric Acid biology business.industry Infantile nystagmus syndrome Optokinetic reflex Syndrome biology.organism_classification Calcium Channel Blockers eye diseases Disease Models Animal Anesthesia 030221 ophthalmology & optometry medicine.symptom business Excitatory Amino Acid Antagonists Nystagmus Congenital 030217 neurology & neurosurgery medicine.drug |
| Zdroj: | Investigative Ophthalmology & Visual Science, 58 (7) |
| ISSN: | 1552-5783 0146-0404 |
| Popis: | Purpose: Infantile nystagmus syndrome (INS) is a disorder characterized by typical horizontal eye oscillations. Due to the uncertain etiology of INS, developing specific treatments remains difficult. Single reports demonstrated, on limited measures, alleviating effects of gabapentin and memantine. In the current study, we employed the zebrafish INS model belladonna (bel) to conduct an in-depth study of how gabapentin and memantine interventions alleviate INS signs, which may further restore visual conditions in affected subjects. Moreover, we described the influence of both medications on ocular motor functions in healthy zebrafish, evaluating possible iatrogenic effects. Methods: Ocular motor function and INS characteristics were assessed by eliciting optokinetic response, spontaneous nystagmus, and spontaneous saccades in light and in dark, in 5- to 6-day postfertilization bel larvae and heterozygous siblings. Single larvae were recorded before and after a 1-hour drug treatment (200 mM gabapentin/0.2 mM memantine). Results: Both interventions significantly reduced nystagmus intensity (gabapentin: 59.98%, memantine: 39.59%). However, while the application of gabapentin affected all tested ocular motor functions, memantine specifically reduced nystagmus amplitude and intensity, and thus left controls completely unaffected. Finally, both drug treatments resulted in specific changes in nystagmus waveform and velocity. Conclusions: Our study provides deeper insight into gabapentin and memantine treatment effect in the zebrafish INS model. Moreover, this study should establish zebrafish as a pharmacologic animal model for treating nystagmus and ocular motor disease, serving as a basis for future large-scale drug screenings. Investigative Ophthalmology & Visual Science, 58 (7) ISSN:0146-0404 |
| Databáze: | OpenAIRE |
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