Design and fabrication of a hybrid alginate hydrogel/poly(ε-caprolactone) mold for auricular cartilage reconstruction

Autor: Andrew Gleadall, Gijsje H. Koenderink, Joel Segal, P.P.M. van Zuijlen, Federica Burla, J.K. Buskermolen, Marco N. Helder, Dafydd O. Visscher
Přispěvatelé: Maxillofacial Surgery (VUmc), Plastic, Reconstructive and Hand Surgery, Amsterdam Movement Sciences - Restoration and Development, Dermatology, Oral and Maxillofacial Surgery / Oral Pathology
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Materials science
Alginates
Surface Properties
Polyesters
0206 medical engineering
Biomedical Engineering
Biocompatible Materials
02 engineering and technology
Matrix (biology)
engineering.material
Original Research Report
Biomaterials
chemistry.chemical_compound
Chondrocytes
Tissue engineering
Original Research Reports
biopolymer
polycaprolactone
medicine
Animals
Regeneration
cartilage
Cell Proliferation
Bioprosthesis
Tissue Engineering
Tissue Scaffolds
Cartilage
Goats
tissue scaffold
Hydrogels
3D printing
021001 nanoscience & nanotechnology
Chondrogenesis
020601 biomedical engineering
Biomechanical Phenomena
medicine.anatomical_structure
chemistry
Polycaprolactone
Printing
Three-Dimensional
engineering
Implant
Biopolymer
Ear Cartilage
0210 nano-technology
Caprolactone
Porosity
Biomedical engineering
Zdroj: Journal of Biomedical Materials Research Part B Applied Biomaterials, 107(5), 1711-1721. John Wiley and Sons Inc.
Visscher, D O, Gleadall, A, Buskermolen, J K, Burla, F, Segal, J, Koenderink, G H, Helder, M N & van Zuijlen, P P M 2019, ' Design and fabrication of a hybrid alginate hydrogel/poly(ε-caprolactone) mold for auricular cartilage reconstruction ', Journal of Biomedical Materials Research Part B. Applied Biomaterials, vol. 107, no. 5, pp. 1711-1721 . https://doi.org/10.1002/jbm.b.34264
Journal of Biomedical Materials Research. Part B, Applied Biomaterials
Visscher, D O, Gleadall, A, Buskermolen, J K, Burla, F, Segal, J, Koenderink, G H, Helder, M N & van Zuijlen, P P M 2019, ' Design and fabrication of a hybrid alginate hydrogel/poly(ε-caprolactone) mold for auricular cartilage reconstruction ', Journal of Biomedical Materials Research Part B Applied Biomaterials, vol. 107, no. 5, pp. 1711-1721 . https://doi.org/10.1002/jbm.b.34264
Journal of Biomedical Materials Research Part B. Applied Biomaterials, 107(5), 1711-1721. John Wiley and Sons Inc.
ISSN: 1552-4973
Popis: The aim of this study was to design and manufacture an easily assembled cartilage implant model for auricular reconstruction. First, the printing accuracy and mechanical properties of 3D‐printed poly‐ε‐caprolactone (PCL) scaffolds with varying porosities were determined to assess overall material properties. Next, the applicability of alginate as cell carrier for the cartilage implant model was determined. Using the optimal outcomes of both experiments (in terms of (bio)mechanical properties, cell survival, neocartilage formation, and printing accuracy), a hybrid auricular implant model was developed. PCL scaffolds with 600 μm distances between strands exhibited the best mechanical properties and most optimal printing quality for further exploration. In alginate, chondrocytes displayed high cell survival (~83% after 21 days) and produced cartilage‐like matrix in vitro. Alginate beads cultured in proliferation medium exhibited slightly higher compressive moduli (6 kPa) compared to beads cultured in chondrogenic medium (3.5 kPa, p > .05). The final auricular mold could be printed with 300 μm pores and high fidelity, and the injected chondrocytes survived the culture period of 21 days. The presented hybrid auricular mold appears to be an adequate model for cartilage tissue engineering and may provide a novel approach to auricular cartilage regeneration for facial reconstruction. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 1711–1721, 2019.
Databáze: OpenAIRE
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