Comparison of an immuno-turbidometric method (STalia®d-DI) with an established enzyme linked fluorescent assay (VIDAS®) d-dimer for the exclusion of venous thromboembolism
| Autor: | P. M. Baker, James Beavis, David Keeling, K. Sukhu |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
medicine.medical_specialty
business.industry Biochemistry (medical) Clinical Biochemistry Enzyme-Linked Immunosorbent Assay Venous Thromboembolism Hematology General Medicine Economic benefits Surgery Fibrin Fibrinogen Degradation Products Clinical Practice Nephelometry and Turbidimetry Predictive Value of Tests Internal medicine D-dimer Outpatient setting Humans Medicine Fibrinogen Equivalent Unit Ultrasonography business Venous thromboembolism Latex Fixation Tests |
| Zdroj: | International Journal of Laboratory Hematology. 30:200-204 |
| ISSN: | 1751-553X 1751-5521 |
| DOI: | 10.1111/j.1751-553x.2007.00942.x |
| Popis: | The use of d-dimer tests for the exclusion of venous thromboembolism is an important advance in clinical practice and also has economic benefits. The Stalia D-Di (Diagnostica Stago, Asnieres, France) is a semi automated system for the quantification of d-dimer using an immuno-turbidometric method incorporating a suspension of latex microparticles coated with two different monoclonal antibodies specifically targeted against human d-dimer fragments. Results are available rapidly in10 min compared with 35 min for the established VIDAS D-dimer automated enzyme-linked immunosorbent assay (ELISA, BioMerieux, Basingstoke, UK). During November and December 2005, 100 consecutive patients attending the outpatient deep venous thrombosis (DVT) clinic were tested using the VIDAS D-dimer as part of the routine DVT investigation. Using the same samples, D-dimer estimation was also performed on the STalia D-Di for comparison. Across a wide range of data (Vidas 83-5656) and (STali200-4000), there was good agreement between the two methods. Using cutoff's of 500 microg/l fibrinogen equivalent units (Keeling et al., 1999), 42% (42/100) patients were negative (500) and 46% (46/100) were positive (500) on both systems. Six per cent (6/100) were positive on the Vidas but negative on the STalia and another 6% (6/100) were positive on the STalia but negative on the Vidas. In conclusion, 88% (88/100) of patients showed agreement and in the other 12% (12/100), one had a DVT as identified by Compression ultrasonography (CUS). In this study, there were seven patients with a DVT as identified by CUS and they all scored as 'likely' on a pretest clinical probability score and so negative D-dimer would not be used clinically to rule out the disease. The Vidas is a well established instrument for D-dimer measurement in outpatient DVT clinics, and in this small study the STalia compares very well and therefore would fit into an outpatient setting for D-dimer measurement. But ideally a larger study would be required before implementing new methodology in a clinical setting. |
| Databáze: | OpenAIRE |
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