Embryology of esophageal atresia in the adriamycin rat model
| Autor: | Juan A. Diez-Pardo, Anne K Possögel, Juan A. Tovar, C. Navarro, C. Morales |
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| Rok vydání: | 1998 |
| Předmět: |
animal structures
Fistula Tracheoesophageal fistula Pregnancy medicine Animals Rats Wistar Esophagus Esophageal Atresia Fetus Antibiotics Antineoplastic business.industry Esophageal disease Abnormalities Drug-Induced Foregut General Medicine Anatomy medicine.disease Rats medicine.anatomical_structure Doxorubicin Embryology Atresia embryonic structures Pediatrics Perinatology and Child Health Female Surgery business Tracheoesophageal Fistula |
| Zdroj: | Journal of Pediatric Surgery. 33:606-612 |
| ISSN: | 0022-3468 |
| DOI: | 10.1016/s0022-3468(98)90326-8 |
| Popis: | Purpose: The aim of this study was to describe the dysmorphogenetic process leading to esophageal atresia and tracheoesophageal fistula (EA + TEF) in the recently developed Adriamycin model of the malformation. Methods: Time-mated pregnant rats were given either Adriamycin (1.75 mg/kg intraperitoneally) or saline on days 6 to 9 of gestation, and their embryos recovered on days 12, 12.5, and 13 were serially sectioned in the transversal plane and studied microscopically after H&E and PAS staining. The findings were compared with those of age-matched untreated embryos. Results: All untreated and saline embryos were normal, whereas 49% of Adriamycin embryos had foregut malformations. Tracheoesophageal separation was complete on day 12 in control embryos, whereas 9 of 10 Adriamycin-exposed embryos had a common esophagotrachea with low emergence of the bronchi at that stage. This pattern had evolved into that of a regular EA + TEF in all nine malformed embryos by day 13. On day 12.5, esophagotrachea was found in 6 of 13 and EA + TEF in 5 of 13 embryos. Two had less well-defined malformations. Conclusions: Esophagotrachea equivalent to complete tracheoesophageal cleft is the first step leading to EA + TEF in this model. The full-blown malformation is finally acquired by partial loss of the posterior wall of the foregut, which tapers-off in the mediastinal mesenchyme and respiratory differentiation of the anterior wall down to the level of bronchial bifurcation, where it constitutes the fistula and the distal esophagus. |
| Databáze: | OpenAIRE |
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