Translocator protein (18 kDa) regulates the microglial phenotype in Parkinson’s disease through P47

Autor:	Xue, Xue, Rui, Duan, Guoyan, Zheng, Hucheng, Chen, Weiwei, Zhang, Liang, Shi
Rok vydání:	2022
Předmět:	Lipopolysaccharides Phenotype Receptors GABA Humans Parkinson Disease Bioengineering Microglia General Medicine Ligands Applied Microbiology and Biotechnology Adaptor Proteins Signal Transducing Biotechnology
Zdroj:	Bioengineered. 13:11062-11072
ISSN:	2165-5987 2165-5979
Popis:	Numerous studies have suggested that the phenotypic transformation of microglia plays a role in the pathogenesis of Parkinson's disease (PD). Translocator protein (TSPO) is an 18 kDa translocator membrane protein that acts as a marker of neuroinflammation and suppresses neuroinflammation; however, its underlying mechanism remains unclear. Although TSPO ligands were found to be protective in several neurodegenerative paradigms, few studies have evaluated their effects on microglial polarization, and underlying mechanisms need to be explored. In the present study, we examined the effects of TSPO and PK11195, a TSPO ligand, on lipopolysaccharide (LPS)+interferon (IFN)-γ-induced inflammatory factors and oxidative stress in microglia using enzyme-linked immunosorbent assay. The effect of TSPO and PK11195 on LPS+IFN-γ-induced microglial cell apoptosis was examined using immunofluorescence (IF), flow cytometry, and western blotting. The interaction between TSPO and P47 was investigated using IF and co-immunoprecipitation analysis.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d7cabe84b46071bfa90ff4a54c421893 https://doi.org/10.1080/21655979.2022.2068754 Zobrazit plný text záznamu