Polymer fraction of Aloe vera exhibits a protective activity on ethanol-induced gastric lesions
| Autor: | Chul-Hong Park, Sang-Han Lee, Si-Rim Lee, Jin-Hong Baek, Tae-Yang Cha, Hyeong-U Son, Hyun-Jin Lee, Dong-Yoon Nam, Jin-Chul Heo |
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| Rok vydání: | 2010 |
| Předmět: |
Male
Polymers Pharmacology Matrix metalloproteinase Aloe vera Mice Genetics Gastric mucosa Medicine Animals Stomach Ulcer Aloe Inflammation Messenger RNA Mice Inbred BALB C biology Oncogene Ethanol business.industry Gene Expression Profiling General Medicine biology.organism_classification Molecular medicine Nitric oxide synthase medicine.anatomical_structure Matrix Metalloproteinase 9 Apoptosis Gastric Mucosa Immunology biology.protein Plant Preparations Nitric Oxide Synthase business Biomarkers |
| Zdroj: | International journal of molecular medicine. 27(4) |
| ISSN: | 1791-244X |
| Popis: | For centuries, Aloe has been used as a herbal plant remedy against skin disorders, diabetes, and for its cardiac stimulatory activity. Here, we examined the gastroprotective effects of an Aloe vera polymer fraction (Avpf; molecular weight cut-off ≥50 kDa; 150 mg/kg body weight, p.o.) on an ethanol-induced gastric lesion mouse model. Mice pre-treated with Avpf had significantly fewer gastric lesions than their respective controls. To further examine the potential mechanism underlying this effect, we used reverse transcription-polymerase chain reaction to examine nitric oxide synthase and matrix metalloproteinase (MMP)mRNA expression on tissues from gastric lesions. Our results revealed that the mRNA expressions of inducible nitric oxide synthase (iNOS) and neuronal nitric oxide synthase (nNOS) were each reduced by ~50% in Avpf-treated mice vs. the controls, whereas, the mRNA expression levels of endothelial nitric oxide synthase remained unchanged. MMP-9, an index for gastric lesions, also alleviated the ethanol-treated gastric ulceration during Avpf treatment. These findings collectively suggest that Avpf significantly protects the gastric mucosa against ethanol-induced gastric damage, at least in part, by decreasing mRNA expression levels of not only iNOS and nNOS, but also MMP-9. |
| Databáze: | OpenAIRE |
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