The cyanobacterial oligopeptides microginins induce DNA damage in the human hepatocellular carcinoma (HepG2) cell line
| Autor: | Metka Filipič, Milán Riba, Andrea Zsuzsanna Ujvárosi, Gábor Vasas, Bojana Žegura, Sándor Gonda, Klara Hercog |
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| Rok vydání: | 2020 |
| Předmět: |
Carcinoma
Hepatocellular Environmental Engineering DNA damage Health Toxicology and Mutagenesis 0208 environmental biotechnology 02 engineering and technology 010501 environmental sciences Cyanobacteria medicine.disease_cause 01 natural sciences Genomic Instability chemistry.chemical_compound medicine Humans Environmental Chemistry MTT assay Cytotoxicity 0105 earth and related environmental sciences Oligopeptide Micronucleus Tests Liver Neoplasms Public Health Environmental and Occupational Health Hep G2 Cells General Medicine General Chemistry Pollution Molecular biology 020801 environmental engineering chemistry Cell culture Comet Assay Micronucleus Oligopeptides Genotoxicity DNA DNA Damage |
| Zdroj: | Chemosphere. 240:124880 |
| ISSN: | 0045-6535 |
| DOI: | 10.1016/j.chemosphere.2019.124880 |
| Popis: | Microginins (MGs) are bioactive metabolites mainly produced by Microcystis spp., (Cyanobacteria) commonly found in eutrophic environments. In this study, the cytotoxic and genotoxic activities of four MG congeners (MG FR3, MG GH787, cyanostatin B, MGL 402) and a well characterized cyanobacterial extract B-14-01 containing these metabolites were evaluated in the human hepatocellular carcinoma (HepG2) cell line. The cytotoxicity was measured with the MTT assay, while genotoxicity was studied with the comet, γH2AX and cytokinesis block (CBMN) micronucleus assays. The viability of cells after 24 h was significantly affected only by the extract, whereas after 72 h a concentration dependent decrease in cell proliferation was observed for the extract and tested microginins, with MGL 402 being the most potent and MG FR3 the least potent congener. The extract and all tested congeners induced DNA strand breaks after 4 and 24 h exposure. The most potent was the extract, which induced concentration and time dependent increase in DNA damage at concentrations ≥0.01 μg mL−1. Among microginins the most potent was MGL 402 (increase in DNA strand breaks at ≥ 0.01 μg mL−1) and MG FR3 was the least potent (increase in DNA strand breaks at ≥ 1 μg mL−1). However, no induction of DNA double strand breaks was observed after 24 and 72-h exposure to the cyanobacterial extract or MGs. Induction of genomic instability was observed in cells exposed to MG GH787, cyanostatin B and the extract B-14-01. This study is the first to provide the evidence that microginins exert genotoxic activity. |
| Databáze: | OpenAIRE |
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