Anticancer siRNA cocktails as a novel tool to treat cancer cells. Part (A). Mechanisms of interaction

Autor: Rafael Gomez-Ramirez, Francisco Javier de la Mata, Svetlana Loznikova, Alya G. Venyaminova, Inessa Halets, Jean-Pierre Majoral, Dzmitry Shcharbin, Katarzyna Milowska, D. S. Novopashina, Volha Dzmitruk, Maksim Ionov, Joanna Lazniewska, Olga A. Krasheninina, Maria Bryszewska, Evgeny K. Apartsin, Olga Nowacka
Přispěvatelé: Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, University of Lódź, Institute of Biophysics and Cell Engineering, NASB, Minsk, National Academy of Sciences of Belarus (NASB), Institute of of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Institute of Chemical Biology and Fundamental Medicine, Departamento Química Orgánica y Química Inorgánica, Universidad de Alcalá, Alcalá de Henares, Universidad de Alcalá - University of Alcalá (UAH), Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Instituto de Salud Carlos III [Madrid] (ISC)-ministerio de ciencia e innovacion, Laboratoire de chimie de coordination (LCC), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie de Toulouse (ICT-FR 2599), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Ionov, Maksim, Lazniewska, Joanna, Dzmitruk, Volha, Halets, Inessa, Loznikova, Svetlana, Novopashina, Darya, Apartsin, Evgeny, Krasheninina, Olga, Venyaminova, Alya, Milowska, Katarzyna, Nowacka, Olga, Gomez-Ramirez, Rafael, Javier de la Mata, Francisco, Majoral, Jean-Pierre, Shcharbin, Dzmitry, Bryszewska, Maria
Rok vydání: 2015
Předmět:
Dendrimers
Small interfering RNA
Circular dichroism
Light
RNase P
Stereochemistry
anticancer siRNA
biophysical characteristics
bcl-X Protein
Pharmaceutical Science
02 engineering and technology
dendrimer
Transfection
03 medical and health sciences
Microscopy
Electron
Transmission
complex formation
Neoplasms
Dendrimer
Zeta potential
Humans
Scattering
Radiation
[CHIM.COOR]Chemical Sciences/Coordination chemistry
Pharmacology & Pharmacy
Particle Size
RNA
Small Interfering
030304 developmental biology
Electrophoresis
Agar Gel
0303 health sciences
Heparin
Chemistry
Circular Dichroism
Phosphorus
Silanes
021001 nanoscience & nanotechnology
3. Good health
Gene Expression Regulation
Neoplastic
RNAi Therapeutics
Spectrometry
Fluorescence
Proto-Oncogene Proteins c-bcl-2
Cell culture
Cancer cell
Biophysics
Myeloid Cell Leukemia Sequence 1 Protein
Nucleic Acid Conformation
Nanocarriers
0210 nano-technology
nano-drug-delivery
Zdroj: International Journal of Pharmaceutics
International Journal of Pharmaceutics, Elsevier, 2015, 485 (1–2), pp.261-269. ⟨10.1016/j.ijpharm.2015.03.024⟩
ISSN: 0378-5173
DOI: 10.1016/j.ijpharm.2015.03.024
Popis: International audience; This paper examines a perspective on the use of newly engineered nanomaterials as effective and safe carriers of genes for the therapy of cancer. Three different groups of cationic dendrimers (PAMAM, phosphorus and carbosilane) were complexed with anticancer siRNA and their biophysical properties of the dendriplexes analyzed. The potential of the dendrimers as nanocarriers for anticancer siBcl-xl, siBcl-2, siMcl-1 siRNAs and a siScrambled sequence was explored. Dendrimer/siRNA complexes were characterized by methods including fluorescence, zeta potential, dynamic light scattering, circular dichroism, gel electrophoresis and transmission electron microscopy. Some of the experiments were done with heparin to check if siRNA can be easily disassociated from the complexes, and whether released siRNA maintains its structure after interaction with the dendrimer. The results indicate that siRNAs form complexes with all the dendrimers tested. Oligoribonucleotide duplexes can be released from dendriplexes after heparin treatment and the structure of siRNA is maintained in the case of PAMAM or carbosilane dendrimers. The dendrimers were also effective in protecting siRNA from RNase A activity. The selection of the best siRNA carrier will be made based on cell culture studies (Part B).
Databáze: OpenAIRE
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