Identification and Functional Analysis of microRNAs Involved in the Anther Development in Cotton Genic Male Sterile Line Yu98-8A
| Autor: | Fu’an Zhao, Wang Ye, Xiaojie Yang, Tang Zhongjie, Li Sun, Yao Sun, Zuoren Yang, Nardana Esmaeili, Weiping Fang, Lihong Nie, Shuping Lv, Yuanming Zhao, Deyi Xie, Guo Yin, Neelam Mishra, Wu Li, Wei Zhu, Xunlu Zhu |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Small RNA Sterility Enzyme-Linked Immunosorbent Assay Flowers Biology phytohormone Genes Plant Real-Time Polymerase Chain Reaction Genome cotton Article Catalysis molecular regulation Inorganic Chemistry lcsh:Chemistry 03 medical and health sciences male abortion Flower induction Gene expression microRNA Physical and Theoretical Chemistry Molecular Biology Gene lcsh:QH301-705.5 Spectroscopy Genetics Gossypium Base Sequence Indoleacetic Acids Sequence Analysis RNA Organic Chemistry RNA General Medicine Computer Science Applications MicroRNAs 030104 developmental biology lcsh:Biology (General) lcsh:QD1-999 miRNAs targets Transcriptome Sequence Alignment |
| Zdroj: | International Journal of Molecular Sciences; Volume 17; Issue 10; Pages: 1677 International Journal of Molecular Sciences, Vol 17, Iss 10, p 1677 (2016) International Journal of Molecular Sciences |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms17101677 |
| Popis: | Hybrid vigor contributes in a large way to the yield and quality of cotton (Gossypium hirsutum) fiber. Although microRNAs play essential regulatory roles in flower induction and development, it is still unclear if microRNAs are involved in male sterility, as the regulatory molecular mechanisms of male sterility in cotton need to be better defined. In this study, two independent small RNA libraries were constructed and sequenced from the young buds collected from the sporogenous cell formation to the meiosis stage of the male sterile line Yu98-8A and the near-isogenic line. Sequencing revealed 1588 and 1536 known microRNAs and 347 and 351 novel miRNAs from male sterile and male fertile libraries, respectively. MicroRNA expression profiles revealed that 49 conserved and 51 novel miRNAs were differentially expressed. Bioinformatic and degradome analysis indicated the regulatory complexity of microRNAs during flower induction and development. Further RT-qPCR and physiological analysis indicated that, among the different Kyoto Encyclopedia Gene and Genomes pathways, indole-3-acetic acid and gibberellic acid signaling transduction pathways may play pivotal regulatory functions in male sterility. |
| Databáze: | OpenAIRE |
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