The use of human recombinant gonadotropin receptors to search for immunoglobulin G-mediated premature ovarian failure
| Autor: | M R Flack, Lawrence M. Nelson, J N Anasti, Jeffrey P. Froehlich |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Adult
medicine.medical_specialty Adolescent endocrine system diseases medicine.drug_class Endocrinology Diabetes and Metabolism Clinical Biochemistry Primary Ovarian Insufficiency Biochemistry Immunoglobulin G Mice Endocrinology Internal medicine Blocking antibody Cyclic AMP Tumor Cells Cultured medicine Animals Humans Receptor biology Biochemistry (medical) Transfection Luteinizing Hormone Receptors LH Recombinant Proteins Cell culture Polyclonal antibodies biology.protein Receptors FSH Female Follicle Stimulating Hormone Antibody Gonadotropin |
| Zdroj: | The Journal of Clinical Endocrinology & Metabolism. 80:824-828 |
| ISSN: | 1945-7197 0021-972X |
| DOI: | 10.1210/jcem.80.3.7883837 |
| Popis: | Anti-FSH receptor antibodies, detected using animal systems, have been reported in a few patients with premature ovarian failure (POF). However, assays based on animal receptors may be inappropriate for detecting inhibiting antibodies in humans. Accordingly, we tested for interfering antibodies in patients with POF using a recombinant system expressing human (h) FSH and LH receptors. A mouse adrenal cell line transfected with the hFSH receptor (Y1-hFSHR) exhibits a dose-dependent increase in progesterone when exposed to hFSH. An embryonal kidney cell line transfected with the hLH receptor gene (hLHR-293) exhibits a dose-dependent increase in cAMP when exposed to hLH. We isolated immunoglobulins G (IgG) from 38 patients with POF and 14 normal women. We stimulated Y1-hFSHR and hLHR-293 cells with hFSH or hLH in the presence of these IgG and determined the resulting progesterone or cAMP response. The progesterone and cAMP responses obtained in the presence of IgG from patients with POF did not differ significantly from the responses in the presence of IgG from normal women. In contrast, antigonadotropin polyclonal antibodies isolated in the same manner as the above IgGs caused a greater than 90% reduction in the response of the Y1-hFSHR and hLHR-293 cells. We did not detect inhibitory antibodies in any of our 38 patients with POF. Therefore, if blocking antibodies interfering with gonadotropin-receptor interaction are a mechanism for POF, they account for a small minority of cases (< 8%). |
| Databáze: | OpenAIRE |
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