Transformation of the white-rot basidiomycete Coriolus hirsutus using the ornithine carbamoyltransferase gene
| Autor: | Yukio Kita, Jun Sugiura, Akira Tsukamoto, Yasushi Kojima |
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| Rok vydání: | 2003 |
| Předmět: |
Mutant
Molecular Sequence Data Gene Dosage Dehydrogenase Biology Applied Microbiology and Biotechnology Biochemistry Chromosomes Analytical Chemistry 3-Isopropylmalate Dehydrogenase chemistry.chemical_compound Transformation Genetic Ornithine Carbamoyltransferase Cloning Molecular Molecular Biology Gene Southern blot Base Sequence Basidiomycota Organic Chemistry General Medicine Protoplast Transformation (genetics) Alcohol Oxidoreductases Blotting Southern chemistry DNA Biotechnology |
| Zdroj: | Bioscience, biotechnology, and biochemistry. 67(10) |
| ISSN: | 0916-8451 |
| Popis: | An efficient transformation system for the basidiomycete Coriolus hirsutus was developed. A double-auxotrophic mutant of C. hirsutus, deficient both in ornithine carbamoyltransferase (OCTase) and 3-isopropylmalate dehydrogenase (3-IPM dehydrogenase), was transformed to Arg+ with each allelic type of the C. hirsutus genomic OCTase gene (arg1) newly cloned. The transformation frequency of 10(3)-10(4) transformants per mug DNA per 10(6)-10(7) oidial protoplasts was reached. Southern blots showed that the transforming DNA was integrated into chromosomal DNA with multi-copies. The Arg+ phenotype of the transformants was stably inherited through mitosis. |
| Databáze: | OpenAIRE |
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