Ultrastructural maturation of the endbulb of Held active zones comparing wild-type and otoferlin-deficient mice
| Autor: | Mehmet Gültas, Carolin Wichmann, Anika Hintze, Esther A. Semmelhack |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
education.field_of_study Multidisciplinary Science Population Wild type Audiology 02 engineering and technology Molecular neuroscience Neurotransmission Biology 021001 nanoscience & nanotechnology Synaptic vesicle Article Cell biology 03 medical and health sciences 030104 developmental biology Cellular neuroscience Cellular Neuroscience OTOF Molecular Neuroscience 0210 nano-technology education Cochlea |
| Zdroj: | iScience iScience, Vol 24, Iss 4, Pp 102282-(2021) |
| ISSN: | 2589-0042 |
| DOI: | 10.1016/j.isci.2021.102282 |
| Popis: | Summary Endbulbs of Held are located in the anteroventral cochlear nucleus and present the first central synapses of the auditory pathway. During development, endbulbs mature functionally to enable rapid and powerful synaptic transmission with high temporal precision. This process is accompanied by morphological changes of endbulb terminals. Loss of the hair cell-specific protein otoferlin (Otof) abolishes neurotransmission in the cochlea and results in the smaller endbulb of Held terminals. Thus, peripheral hearing impairment likely also leads to alterations in the morphological synaptic vesicle (SV) pool size at individual endbulb of Held active zones (AZs). Here, we investigated endbulb AZs in pre-hearing, young, and adult wild-type and Otof−/− mice. During maturation, SV numbers at endbulb AZs increased in wild-type mice but were found to be reduced in Otof−/− mice. The SV population at a distance of 0–15 nm was most strongly affected. Finally, overall SV diameters decreased in Otof−/− animals during maturation. Graphical abstract Highlights • Maturation of wt endbulb of Held active zones leads to more synaptic vesicles • At endbulbs of otoferlin knockout mice, synaptic vesicles decline with age • Mainly two distinct synaptic vesicle populations are affected • Synaptic vesicles sizes are reduced in six-month-old otoferlin knockout animals Audiology; Molecular Neuroscience; Cellular Neuroscience |
| Databáze: | OpenAIRE |
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