Stercorarial Shedding and Transtadial Transmission of Hepatitis B Virus by Common Bed Bugs (Hemiptera: Cimicidae)
| Autor: | Ann L. Silverman, Jamie A. Blow, Michael J. Turell, Edward D. Walker |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Hepatitis B virus
animal structures Cimicidae medicine.disease_cause Polymerase Chain Reaction Sensitivity and Specificity Virus Microbiology Bed bug parasitic diseases medicine Animals Humans General Veterinary biology fungi Blood meal biology.organism_classification Virology Hemiptera Insect Vectors Virus Shedding Blotting Southern Infectious Diseases Hepadnaviridae Insect Science DNA Viral Parasitology Cimex lectularius |
| Zdroj: | Journal of Medical Entomology. 38:694-700 |
| ISSN: | 0022-2585 |
| DOI: | 10.1603/0022-2585-38.5.694 |
| Popis: | Transtadial persistence and stercorarial shedding of hepatitis B virus (HBV) in common bed bugs, Cimex lectularius L., was studied by using experimental infectious blood feedings, infectious intrathoracic inoculations, and virus detection by polymerase chain reaction and Southern hybridization. Results showed that HBV persisted after an infectious blood meal in bed bug bodies for up to 35 d after the infectious blood meal. It was passed transtadially through one molt regardless of instar, was shed in fecal droplets for up to 35 d after the infectious blood meal, but was not passed transovarially. In bugs inoculated intrathoracically, HBV was detected for 21 d postinoculation. Previous studies detected the hepatitis B surface antigen found on both infectious and noninfectious particles in bed bugs. In this study, the presence of nucleic acids amplified from a conserved core region of the viral genome in bodies and feces of C. lectularius suggests that the HBV virus may be mechanically transmitted in feces or when bugs are crushed, during feeding. |
| Databáze: | OpenAIRE |
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