A decisive bridge between innate immunity and the pathognomonic morphological characteristics of type 1 diabetes demonstrated by instillation of heat-inactivated bacteria in the pancreatic duct of rats
| Autor: | Tegehall Angie, Ingvast Sofie, Melhus Åsa, Skog Oskar, Korsgren Olle |
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| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
Inflammation
Innate immunity Hot Temperature Bacteria Endocrinology Diabetes and Metabolism Pancreatic Ducts General Medicine Endocrinology and Diabetes Immunity Innate Rats Islets of Langerhans Endocrinology Diabetes Mellitus Type 1 Endokrinologi och diabetes Internal Medicine Type 1 Diabetes Animals Animal model Acquired immunity Insulitis |
| Popis: | Aims Periductal inflammation and accumulation of granulocytes and monocytes in the periislet area and in the exocrine pancreas is observed within hours after instillation of heat-inactivated bacteria in the ductal compartment of the pancreas in healthy rats. The present investigation was undertaken to study how the acute inflammation developed over time. Methods Immunohistochemical evaluation of the immune response triggered by instillation of heat-inactivated bacteria in the ductal compartment in rats. Results After three weeks, the triggered inflammation had vanished and pancreases showed normal morphology. However, a distinct accumulation of both CD4+ and CD8+ T cells within and adjacent to affected islets was found in one-third of the rats instilled with heat-inactivated E. faecalis, mimicking the insulitis seen at onset of human T1D. As in T1D, this insulitis affected a minority of islets and only certain lobes of the pancreases. Notably, a fraction of the T cells expressed the CD103 antigen, mirroring the recently reported presence of tissue resident memory T cells in the insulitis in humans with recent onset T1D. Conclusions The results presented unravel a previously unknown interplay between innate and acquired immunity in the formation of immunopathological events indistinguishable from those described in humans with recent onset T1D. |
| Databáze: | OpenAIRE |
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