β-lactamase-producing Gram-negative bacteria in an intensive care unit in southern Brazil
| Autor: | Rosilene Fressatti Cardoso, Regiane Bertin de Lima Scodro, Katiany Rizzieri Caleffi-Ferracioli, Clariana Akemi Kariya Leite, Rubia Andreia Falleiros de Pádua, Vera Lúcia Dias Siqueira, Claudia Terencio Agostinho Pires, Karina Yoshimi Oizumi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Bacterial typing Klebsiella pneumoniae 030106 microbiology lcsh:RS1-441 medicine.disease_cause Enterobacter aerogenes Antimicrobial resistance Microbiology Beta-lactamases lcsh:Pharmacy and materia medica 03 medical and health sciences Intergenic region Enterobacteriaceae Genotype medicine General Pharmacology Toxicology and Pharmaceutics Genetic diversity Acinetobacter spp biology Pseudomonas aeruginosa Acinetobacter biology.organism_classification Intensive Care Unit/study/Brazil Drug-resistant bacteria |
| Zdroj: | Brazilian Journal of Pharmaceutical Sciences, Volume: 53, Issue: 2, Article number: e16111, Published: 22 JUN 2017 Brazilian Journal of Pharmaceutical Sciences, Vol 53, Iss 2 (2017) Brazilian Journal of Pharmaceutical Sciences; Vol. 53 No. 2 (2017); e16111 Brazilian Journal of Pharmaceutical Sciences; Vol. 53 Núm. 2 (2017); e16111 Brazilian Journal of Pharmaceutical Sciences; v. 53 n. 2 (2017); e16111 Brazilian Journal of Pharmaceutical Sciences Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 2175-9790 1984-8250 |
| Popis: | The present study evaluated the antimicrobial susceptibility profile, β-lactamase production, and genetic diversity of Enterobacteriaceae, Pseudomonas aeruginosa, and Acinetobacter spp. using phenotypic identification, antimicrobial susceptibility testing, and β-lactamase phenotypic detection. Isolates were obtained from patients in an intensive care unit in a hospital in southern Brazil. Bacterial genomic DNA was extracted, followed by the genotypic detection of carbapenemases and enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR). Fifty-six isolates (26 Klebsiella pneumoniae, five Escherichia coli, three Enterobacter aerogenes, nine P. aeruginosa, and 13 Acinetobacter spp.) were evaluated. The phenotypic extended spectrum β-lactamase (ESBL) test was positive in 53.8% of the K. pneumoniae isolates, 100.0% of the E. coli isolates, and 100.0% of the E. aerogenes isolates. Phenotypic and genotypic testing of K. pneumoniae carbapenemase (KPC) was positive in 50.0% of the K. pneumoniae isolates. Phenotypic and genotypic testing showed that none of the P. aeruginosa or Acinetobacter spp. isolates were positive for metallo- β-lactamase (MBL). The bla OXA gene was detected only in Acinetobacter spp. The lowest genetic diversity, determined by ERIC-PCR, was observed among the KPC-producing K. pneumoniae isolates and OXA-producing Acinetobacter spp. isolates, indicating the inadequate dissemination control of multidrug-resistant bacteria in this hospital environment. |
| Databáze: | OpenAIRE |
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