Sodium bicarbonate cotransporter NBCn1/Slc4a7 affects locomotor activity and hearing in mice
| Autor: | Vibeke Secher Dam, Ebbe Boedtkjer, Donald J. Noble, Sandra M. Garraway, Seongho Bae, Christian Aalkjaer, Kristian Beedholm, Inyeong Choi |
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| Rok vydání: | 2020 |
| Předmět: |
Exploratory Behavior/physiology
Male Vision LOCI Morris water navigation task Open field Behavioral Neuroscience Acid base transporter Mice 0302 clinical medicine Hearing SYNAPTIC PLASTICITY USHER-SYNDROME NMDA RECEPTORS Sodium-Bicarbonate Symporters/genetics ANXIETY Behavior Animal/physiology Mice Knockout 0303 health sciences Retina/metabolism Behavior Animal medicine.anatomical_structure Vision Disorders/genetics ANIMAL-MODELS Knockout mouse Evoked Potentials Auditory Female medicine.symptom NBCN1 SLC4A7 Locomotion medicine.medical_specialty Elevated plus maze GENES Hearing loss Central nervous system Spatial Learning Vision Disorders Biology Mouse behavior Retina 03 medical and health sciences Sodium bicarbonate transporter Emotionality Internal medicine Hearing Loss/genetics medicine Optokinetic drum Animals Hearing Loss 030304 developmental biology SPATIAL MEMORY Sodium-Bicarbonate Symporters AUDITORY IMPAIRMENT Mice Inbred C57BL Locomotion/genetics Endocrinology Evoked Potentials Auditory/genetics Exploratory Behavior Spatial Learning/physiology 030217 neurology & neurosurgery Knockout mice |
| Zdroj: | Choi, I, Beedholm, K, Dam, V S, Bae, S-H, Noble, D J, Garraway, S M, Aalkjær, C & Boedtkjer, E 2021, ' Sodium bicarbonate cotransporter NBCn1/Slc4a7 affects locomotor activity and hearing in mice ', Behavioural Brain Research, vol. 401, 113065 . https://doi.org/10.1016/j.bbr.2020.113065 |
| ISSN: | 1872-7549 |
| Popis: | Despite a widespread expression pattern in the central nervous system, the role of the sodium bicarbonate cotransporter NBCn1/Slc4a7 has not been investigated for locomotor activity, emotion and cognition. Here, we addressed the behavioral consequences of NBCn1 knockout and evaluated hearing and vision that are reportedly impaired in an earlier line of NBCn1 knockout mice and may contribute to behavioral changes. In a circular open field, the knockout mice traveled a shorter distance, especially in the periphery of the chamber, than wildtype littermates. The knockout mice also traveled a shorter total distance in a home cage-like open field. Rearing and grooming behaviors were reduced. The knockout and control mice displayed similar time spent and number of open and closed arms in the elevated plus maze test, indicating negligible change in anxiety. In the Morris water maze test, both groups of mice learned the location of an escape platform within comparable time on the training trials and showed similar platform identification on the probe trial. The knockout mice maintained normal visual responses in the optokinetic drum and produced evoked potentials in response to light stimuli. However, these mice failed to produce auditory evoked potentials. qPCR revealed a robust expression of an alternatively transcribed NBCn1 variant in the knockout mouse retina. These results indicate that NBCn1 deletion leads to reduced locomotor activity in mice by affecting their exploratory behaviors or emotionality. The deletion also causes hearing loss, but its effect on vision varies between different lines of knockout mice. |
| Databáze: | OpenAIRE |
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