An online resource for GPCR structure determination and analysis
| Autor: | Michael A. Hanson, David E. Gloriam, Eshita Mutt, Vignir Isberg, Raymond C. Stevens, Janne M. Bibbe, Tilman Flock, Xavier Deupi, Louise F. Nikolajsen, Christian Munk |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
Rhodopsin
Glycosylation Computer science Allosteric regulation Computational biology Drug action Crystallography X-Ray Protein Engineering Biochemistry Article Receptors G-Protein-Coupled 03 medical and health sciences Resource (project management) Protein Domains Animals Humans Phosphorylation Databases Protein Molecular Biology 030304 developmental biology G protein-coupled receptor Structure (mathematical logic) Internet 0303 health sciences Cryoelectron Microscopy Computational Biology Cell Biology HEK293 Cells Drug Design Mutation Cattle Receptor activation Allosteric Site Software Signal Transduction Biotechnology |
| Zdroj: | Nature Methods |
| ISSN: | 1548-7105 1548-7091 |
| DOI: | 10.1038/s41592-018-0302-x |
| Popis: | G-protein-coupled receptors (GPCRs) transduce physiological and sensory stimuli into appropriate cellular responses and mediate the actions of one-third of drugs. GPCR structural studies have revealed the general bases of receptor activation, signaling, drug action and allosteric modulation, but so far cover only 13% of nonolfactory receptors. We broadly surveyed the receptor modifications/engineering and methods used to produce all available GPCR crystal and cryo-electron microscopy (cryo-EM) structures, and present an interactive resource integrated in GPCRdb ( http://www.gpcrdb.org ) to assist users in designing constructs and browsing appropriate experimental conditions for structure studies. An interactive online resource integrated in the GPCRdb hub presents tools to design GPCR constructs and determine appropriate experimental conditions for structural studies by crystallography and cryo-EM. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|