Electrochemical detection of E. coli 16S rDNA sequence using air-plasma-activated fullerene-impregnated screen printed electrodes
| Autor: | Kazuyoshi Takagi, Tomohito Itoh, Hiroki Hayashi, Tatsuhiko Mori, Joseph Wang, Masayasu Sakane, Haruki Shiraishi |
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| Rok vydání: | 2007 |
| Předmět: |
DNA
Bacterial Hot Temperature Fullerene Base Pair Mismatch Surface Properties Biophysics Analytical chemistry medicine.disease_cause DNA Ribosomal Redox indicator chemistry.chemical_compound RNA Ribosomal 16S Escherichia coli Electrochemistry medicine Physical and Theoretical Chemistry Electrodes In Situ Hybridization Chemistry Oligonucleotide Air Substrate (chemistry) Sequence Analysis DNA General Medicine Screen printing Electrode Fullerenes DNA |
| Zdroj: | Bioelectrochemistry. 70:481-487 |
| ISSN: | 1567-5394 |
| Popis: | A new DNA modified electrode for the electrochemical detection of 16S rDNA extracted from Escherichia coli (JCM1649) is proposed. The electrodes were fabricated by screen printing a fullerene-impregnated carbon ink onto a poly(methylmethacrylate) substrate and immobilizing a probe DNA on the surface after activating the electrode with air plasma. The results indicated a dramatic improvement in the surface coverage of the immobilized probe DNA, and of the reduction peak of the redox indicator (Co(phen)(3)(3+)) due to the incorporation of fullerene. By immobilizing the probe onto the fullerene-impregnated screen-printed electrodes, the PCR product of the 16S rDNA extracted from E. coli was directly detected without any pretreatment. A well defined signal difference was observed between the perfectly matching oligonucleotide and the mismatching one, and it was possible to detect the target at the modified electrode. This method enabled us to clearly detect the two base mismatches in the ca. 1500-bases long 16S rDNA sequence. |
| Databáze: | OpenAIRE |
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