ALTERATION IN HORMONAL ACTIVITY RESULTING FROM CHEMICAL TREATMENT OF BEEF GROWTH HORMONE

Autor:	Martin Sonenberg, William L. Money
Rok vydání:	1960
Předmět:	medicine.medical_specialty Hormonal activity Human Growth Hormone Chemical treatment Chemistry Endocrinology Diabetes and Metabolism General Medicine Growth hormone Endocrinology Growth Hormone Internal medicine medicine Humans Indicators and Reagents
Zdroj:	Acta Endocrinologica. :1-9
ISSN:	1479-683X 0804-4643
DOI:	10.1530/acta.0.xxxiii0001
Popis:	The effect of various reagents, which produce protein derivatives, on the biological activity of growth hormone has been studied. Various conditions which affect growth hormone stability have also been investigated. In general, it has been found that reaction of growth hormone with phenylisocyanate, nitrous acid or concentrated sulfuric acid, under the conditions specified, destroyed the biological activity. Reaction of growth hormone with thioglycolic acid, iodine, or acid methanol yielded growth hormone derivatives which were active or inactive, depending on the reaction conditions. Reaction with acid ethanol, formaldehyde, cysteine, sodium bisulfite, diisopropylfluorophosphate, urea or bromine had no effect on the growth-promoting activity of these preparations. Growth hormone was found to be rather stable in dilute and glacial acetic acid except when heated to a temperature of 94° C. The implications of these findings are discussed. None of the growth hormone derivatives obtained in this study was able to inhibit the growth-promoting response induced by unmodified growth hormone preparations.
Databáze:	OpenAIRE
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