A ROLE FOR INTERLEUKIN 8 IN DIRECT REGULATION OF T CELL FUNCTIONAL ACTIVITY
| Autor: | V. A. Shmarov, Galina V. Seledtsova, M. E. Meniailo, V. V. Malashchenko, Andrei G. Goncharov, O. B. Melashchenko, N. D. Gasatova, Victor I. Seledtsov |
|---|---|
| Jazyk: | ruština |
| Rok vydání: | 2017 |
| Předmět: |
medicine.medical_specialty
T cell CD3 Immunology t lymphocytes interleukin 8 receptor Biology activation of lymphocytes 01 natural sciences 010104 statistics & probability 03 medical and health sciences Interleukin 21 0302 clinical medicine Internal medicine medicine Immunology and Allergy Cytotoxic T cell 030212 general & internal medicine IL-2 receptor 0101 mathematics Effector interleukin-8 CD28 RC581-607 Molecular biology cytokines Endocrinology medicine.anatomical_structure biology.protein Interleukin 12 functional activity Immunologic diseases. Allergy |
| Zdroj: | Medicinskaâ Immunologiâ, Vol 19, Iss 5, Pp 529-536 (2017) |
| ISSN: | 1563-0625 |
| Popis: | CD3+T lymphocytes were isolated from normal donors by positive magnetic separation. Activation of the T cells with particles conjugated with antibodies to CD3, СD28 and СD2 molecules led to substantial increase in T cell production of interleukin-8 (IL-8). An interleukin-8 receptor (CXCR1, CD181) was initially expressed in 13.3% of T lymphocytes. Activation of T lymphocytes resulted into a detectable increase of CD181+ cell number among CD4+ naive cells and CD4+ terminally-differentiated effector cells, and, conversely, into decrease of their number among CD4+ effector memory cells. Activation of T lymphocytes was assessed by membrane expression of CD25 molecule (receptor for IL-2). IL-8 (0.01-10.0 ng/ml) was shown to markedly reduce activation of both CD4- and CD4+ effector memory T cells, as well as terminallydifferentiated T effectors, without significantly affecting activation of naive T lymphocytes and central memory T cells. IL-8 noticeably increased IL-2 production by activated Т cells, caused a reduced IL-10 production, and did not significantly affect the secretion of IFNγ and IL-4. The data obtained suggest a significance of IL-8 for direct regulation of adaptive T cell responses. |
| Databáze: | OpenAIRE |
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