Low expression of Rap1GAP is associated with epithelial-mesenchymal transition (EMT) and poor prognosis in gastric cancer
| Autor: | Jizhao Wang, Xiaoyi Duan, Min Li, Jiansheng Wang, Jia Zhang, Kai Sun, Ya Yang, Yan Yan, Hui Cai, Xu Liu |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Oncology Male Pathology MMP2 Time Factors Kaplan-Meier Estimate Shelterin Complex Metastasis 0302 clinical medicine Cell Movement Tumor size Middle Aged Cadherins Tumor Burden Gene Expression Regulation Neoplastic Matrix metalloproteinase-2 Cardiothoracic surgery 030220 oncology & carcinogenesis Matrix Metalloproteinase 2 Female Signal Transduction Research Paper medicine.medical_specialty Poor prognosis Epithelial-Mesenchymal Transition Telomere-Binding Proteins Down-Regulation Transfection 03 medical and health sciences Antigens CD Stomach Neoplasms Internal medicine Cell Line Tumor medicine Humans Neoplasm Invasiveness Epithelial–mesenchymal transition Neoplasm Staging Proportional Hazards Models business.industry Tumor Suppressor Proteins gastric cancer RAP1 GTPase activating protein (Rap1GAP) Cancer E-cadherin medicine.disease 030104 developmental biology HEK293 Cells Ectopic expression prognosis business |
| Zdroj: | Oncotarget |
| ISSN: | 1949-2553 |
| Popis: | // Ya Yang 1, 4, * , Jia Zhang 1, * , Yan Yan 1 , Hui Cai 2 , Min Li 1 , Kai Sun 1 , Jizhao Wang 1 , Xu Liu 1 , Jiansheng Wang 1 , Xiaoyi Duan 3 1 The Second Department of Thoracic Surgery, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an 710061, Shaanxi, China 2 Department of Vascular Surgery, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an 710061, Shaanxi, China 3 Department of Radiology, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an 710061, Shaanxi, China 4 Department III of Radiation Oncology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450000, Henan, China * These authors have contributed equally to this work Correspondence to: Xiaoyi Duan, email: duanxy@mail.xjtu.edu.cn Jiansheng Wang, email: wangjsh@mail.xjtu.edu.cn Keywords: gastric cancer, RAP1 GTPase activating protein (Rap1GAP), E-cadherin, Matrix metalloproteinase-2, prognosis Received: October 19, 2015 Accepted: November 21, 2016 Published: December 21, 2016 ABSTRACT Rap1GAP is a crucial tumor suppressor, but its role in gastric cancer (GC) is little investigated. In this study, we found that the expression of Rap1GAP was decreased in GC. Low expression of Rap1GAP was positively correlated with advanced pTNM stage, Borrmann types, tumor diameter and poor prognosis in patients with GC. Low expression of Rap1GAP correlated with loss of E-cadherin expression, and anomalous positivity of MMP2 expression. Multivariate analysis showed that low expression of Rap1GAP was an independent prognostic factor. Ectopic expression of Rap1GAP impaired cell migration and invasion, promoted the expression of E-cadherin and decreased the expression of MMP2. These results suggest that Rap1GAP functions as a novel suppressor of EMT and tumor metastasis in GC, and loss of Rap1GAP predicts poor prognosis in GC. |
| Databáze: | OpenAIRE |
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