Antisense-mediated S-adenosyl-L-methionine decarboxylase silencing affects heat stress responses of tobacco plants
| Autor: | Kalliopi A. Roubelakis-Angelakis, Katerina Karamanoli, Ifigeneia Mellidou, Helen-Isis A. Constantinidou |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Carboxy-Lyases Nicotiana tabacum Transgene Plant Science 01 natural sciences 03 medical and health sciences Downregulation and upregulation Gene Expression Regulation Plant Tobacco Gene silencing chemistry.chemical_classification biology fungi food and beverages Hydrogen Peroxide biology.organism_classification Apoplast Cell biology Plant Breeding 030104 developmental biology Enzyme chemistry Agronomy and Crop Science Polyamine oxidase Homeostasis Heat-Shock Response 010606 plant biology & botany |
| Zdroj: | Functional plant biology : FPB. 47(7) |
| ISSN: | 1445-4416 |
| Popis: | Understanding the molecular mode(s) of plant tolerance to heat stress (HS) is crucial since HS is a potential threat to sustainable agriculture and global crop production. Polyamines (PAs) seem to exert multifaceted effects in plant growth and development and responses to abiotic and biotic stresses, presumably via their homeostasis, chemical interactions and contribution to hydrogen peroxide (H2O2) cellular ‘signatures’. Downregulation of the apoplastic POLYAMINE OXIDASE (PAO) gene improved thermotolerance in tobacco (Nicotiana tabacum L.) transgenics. However, in the present work we show that transgenic tobacco plants with antisense-mediated S-ADENOSYL-L-METHIONINE DECARBOXYLASE silencing (AS-NtSAMDC) exhibited enhanced sensitivity and delayed responses to HS which was accompanied by profound injury upon HS removal (recovery), as assessed by phenological, physiological and biochemical characteristics. In particular, the AS-NtSAMDC transgenics exhibited significantly reduced rate of photosynthesis, as well as enzymatic and non-enzymatic antioxidants. These transgenics suffered irreversible damage, which significantly reduced their growth potential upon return to normal conditions. These data reinforce the contribution of increased PA homeostasis to tolerance, and can move forward our understanding on the PA-mediated mechanism(s) conferring tolerance to HS that might be targeted via traditional or biotechnological breeding for developing HS tolerant plants. |
| Databáze: | OpenAIRE |
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