Comparison of glass slides and various digital-slide modalities for cytopathology screening and interpretation
Autor: | Juan Xing, Sara E. Monaco, Liron Pantanowitz, Jacqueline Cuda, Matthew G. Hanna, Ishtiaque Ahmed |
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Rok vydání: | 2017 |
Předmět: |
0301 basic medicine
Cancer Research Scanner Pathology medicine.medical_specialty Cytodiagnosis Magnification 03 medical and health sciences 0302 clinical medicine Neoplasms Image Processing Computer-Assisted Medicine Humans Computer vision Microscopy Modalities Pixel Digital mapping business.industry Digital imaging Digital pathology Reproducibility of Results 030104 developmental biology Oncology Cytopathology 030220 oncology & carcinogenesis Female Artificial intelligence business Software Papanicolaou Test |
Zdroj: | Cancer cytopathology. 125(9) |
ISSN: | 1934-6638 |
Popis: | BACKGROUND Whole-slide imaging in cytology is limited when glass slides are digitized without z-stacks for focusing. Different vendors have started to provide z-stacking solutions to overcome this limitation. The Panoptiq imaging system allows users to create digital files combining low-magnification panoramic images with regions of interest (ROIs) that are imaged with high-magnification z-stacks. The aim of this study was to compare such panoramic images with conventional whole-slide images and glass slides for the tasks of screening and interpretation in cytopathology. METHODS Thirty glass slides, including 10 ThinPrep Papanicolaou tests and 20 nongynecologic cytology cases, were digitized with an Olympus BX45 integrated microscope with an attached Prosilica GT camera. ViewsIQ software was used for image acquisition and viewing. These glass slides were also scanned on an Aperio ScanScope XT at ×40 (0.25 μm/pixel) with 1 z-plane and were viewed with ImageScope software. Digital and glass sides were screened and dotted/annotated by a cytotechnologist and were subsequently reviewed by 3 cytopathologists. For panoramic images, the cytotechnologist manually created digital maps and selected representative ROIs to generate z-stacks at a higher magnification. After 3-week washout periods, panoramic images were compared with Aperio digital slides and glass slides. RESULTS The Panoptiq system permitted fine focusing of thick smears and cell clusters. In comparison with glass slides, the average screening times were 5.5 and 1.8 times longer with Panoptiq and Aperio images, respectively, but this improved with user experience. There was no statistical difference in diagnostic concordance between all 3 modalities. Users' diagnostic confidence was also similar for all modalities. CONCLUSIONS The Aperio whole-slide scanner with 1 z-plane scanning and the Panoptiq imaging system with z-stacking are both suitable for cytopathology screening and interpretation. However, ROI z-stacks do offer a superior mechanism for overcoming focusing problems commonly encountered with digital cytology slides. Unlike whole-slide imaging, the acquisition of representative z-stack images with the Panoptiq system requires a trained cytologist to create digital files. Cancer Cytopathol 2017. © 2017 American Cancer Society. |
Databáze: | OpenAIRE |
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