Bioactive potency of epidermal mucus extracts from greasy grouper, Epinephelus tauvina (Forsskal, 1775)
| Autor: | George Edward, Ganesh Manikantan, Syed Ajmal Khan, Somasundarannair Lyla, Gnana Jothi, Packiaraj Vijayanand |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
High-performance liquid chromatography 03 medical and health sciences Potency Agar diffusion test Proximate analysis lcsh:QH301-705.5 Gel electrophoresis chemistry.chemical_classification lcsh:R5-920 Chromatography biology Epinephelus tauvina Haemolytic assay Fatty acid biology.organism_classification Antimicrobial Mucus 030104 developmental biology lcsh:Biology (General) chemistry Mucus extracts Antimicrobial peptides lcsh:Medicine (General) SDS-PAGE |
| Zdroj: | Journal of Coastal Life Medicine, Vol 4, Iss 7, Pp 510-520 (2016) |
| ISSN: | 2309-6152 2309-5288 |
| DOI: | 10.12980/jclm.4.2016j6-34 |
| Popis: | Objective: To study the bio-potency of epidermal mucus from Epinephelus tauvina. Methods: Mucus was extracted with acidic, organic and aqueous solvents. Protein, carbohydrate, lipid, amino acid and fatty acid content of mucus extracts were quantified by UV-spectrophotometer, high performance liquid chromatography and gas chromatography- mass spectrometer, respectively. Antimicrobial activity was tested against five human and fish pathogens by using agar well diffusion method. The molecular weight of peptides was determined using sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The haemolytic activity of extracts was tested against chick, goat, cow and human red blood cell. Results: Protein contributed with maximum of 26.25% in crude mucus. Arginine was recorded maximum of (133.9 nmol/mL) in crude mucus. 2,4,6-Decatrienoic acid and bis (a-chloroethyl) sulfone were confirmed in organic extract. The antimicrobial activity of acidic extract was significant. Among the human pathogens, maximum zone of inhibition ((26.0 ± 0.3) mm) was observed against Proteus mirabilis. Whereas, among fish pathogens maximum zone of inhibition ((25.0 ± 0.1) mm) was observed against Vibrio parahemolyticus. The activity of other two extracts was not remarkable. The molecular weight of peptides ranged from 115.5- 37.1 kDa in acidic extract was determined. Chicken and goat blood were found to be highly vulnerable to the lysis. Conclusions: The whole mucus could be a promising source with numerous bioactive- potency. Consequently, this preliminary information suggested that mucus is a source of novel antimicrobial agents for fish and human health related applications. |
| Databáze: | OpenAIRE |
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